Biochemical characterisation of the SWI/SNF family member HLTF

被引：34
作者：
MacKay, Craig [1 ]
Toth, Rachel [1 ]
Rouse, John [1 ]
机构：
[1] Univ Dundee, Coll Life Sci, MRC Prot Phosphorylat Unit, Dundee DD1 5EH, Scotland
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 390卷 / 02期
基金：
英国医学研究理事会;
关键词：
HLTP; SWI/SNF; ATPase; Cancer; Ubiquitin; E3; CELL NUCLEAR ANTIGEN; GENOMIC INSTABILITY; UBIQUITIN LIGASE; HUMAN SHPRH; TRANSCRIPTION; HELICASE; UBC13; GENE;
D O I：
10.1016/j.bbrc.2009.08.151
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
HLTF is highly similar in domain organisation to yeast Rad5. We identify PTIP and RPA70, both involved in DNA replication and DNA repair, as HLTF-interacting proteins although cells depleted of HLTF did not show defects in cellular responses to DNA damage. In vitro, HLTF has ATPase activity and E3 ubiquitin ligase activity with a range of E2 ubiquitin conjugating enzymes. HLTF expression is severely reduced in a range of cancer cells, and we suggest that the HLTF antibodies generated in this study could be used for cancer diagnostic purposes. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码：187 / 191
页数：5
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