Evaluation of a commercial MRSA assay when multiple MRSA strains are causing epidemics

被引:11
作者
Sissonen, S. [1 ]
Pasanen, T. [1 ]
Salmenlinna, S. [2 ]
Vuopio-Varkila, J. [2 ]
Tarkka, E. [1 ]
Vaara, M. [1 ]
Tissari, P. [1 ]
机构
[1] Helsinki Univ Hosp, HUSLAB, Div Clin Microbiol, Dept Bacteriol, Helsinki 00029, Finland
[2] Natl Publ Hlth Inst, SF-00300 Helsinki, Finland
关键词
RESISTANT STAPHYLOCOCCUS-AUREUS; IDENTIFICATION;
D O I
10.1007/s10096-009-0771-z
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Rapid and reliable diagnostic methods are needed to control methicillin-resistant Staphylococcus aureus (MRSA) transmission. We studied the BD GeneOhm (TM) MRSA Assay which is based on one specific amplification product at the junction of the right extremity sequence of the staphylococcal cassette chromosome mec (SCCmec) and the chromosomal sequence of orfX of S. aureus. The test was applied on 95 clinical isolates in Finland: 83% were positive. The isolates giving negative results represented several pulsed-field gel electrophoresis (PFGE) types and harboured SCCmec types IV, V, VI or were new types with different combinations of ccr genes.
引用
收藏
页码:1271 / 1273
页数:3
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