Horizontal transfer and hypovirulence associated with double-stranded RNA in Beauveria bassiana

被引：29

作者：

Dalzoto, Patricia R.

Glienke-Blanco, Chirlei

Kava-Cordeiro, Vanessa

Ribeiro, Juliana Z.

Kitajima, Elliot Watanabe

Azevedo, Joao Lucio

机构：

[1] Univ Fed Parana, Dept Patol Basica, BR-81531990 Curitiba, Parana, Brazil

[2] Univ Fed Parana, Dept Genet, BR-81531990 Curitiba, Parana, Brazil

[3] Univ Sao Paulo, ESALQ, BR-13400970 Sao Paulo, Brazil

来源：

MYCOLOGICAL RESEARCH | 2006年 / 110卷

关键词：

biocontrol; dsRNA curing; entomopathogenic fungi; MET; mycoviruses;

D O I：

10.1016/j.mycres.2006.08.009

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Beauveria bassiana strains from different hosts and geographic origins were assayed for the presence of double-stranded RNA (dsRNA). Two of them (15.4%) showed extra bands, with approximately 4.0-3.5 kb and 2-0.7 kb, respectively, after electrophoretic separation of undigested nucleic acids. Virus-like particles were approximately 28-30 nm diam. The dsRNA was maintained after conidiogenesis (vertical transmission) and was transmitted horizontally by hyphal anastomosis. Strains purged of dsRNA obtained after cycloheximide treatment showed increased conidial production when compared with strains carrying dsRNA particles. Bioassays demonstrated hypovirulence associated with dsRNA. The mean mortality against the insect Euschistus heros was reduced in strains containing dsRNA when compared with the isogenic dsRNA-free ones. (c) 2006 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

引用

页码：1475 / 1481

页数：7

共 43 条

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