Hybridization of DNA to bead-immobilized probes confined within a microfluidic channel

被引：32
作者：
Kim, Joohoon [1 ]
Heo, Jinseok [1 ]
Crooks, Richard M. [1 ]
机构：
[1] Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA
来源：
LANGMUIR | 2006年 / 22卷 / 24期
关键词：
D O I：
10.1021/la0616956
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report the factors influencing the capture of DNA by DNA-modified microbeads confined within a microfluidic channel. Quantitative correlation of target capture efficiency to probe surface concentration, solution flow rate, and target concentration are discussed. The results indicate that the microfluidic system exhibits a limit of detection of similar to 10(-10) M (similar to 10(-16) mol) DNA and a selectivity factor of similar to 8 x 10(3). Typical hybridization times are on the order of minutes.
引用
收藏
页码：10130 / 10134
页数：5
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