Transformation of Hibiscus rosa-sinensis L. by Agrobacterium rhizogenes

被引:7
|
作者
Christensen, Brian [1 ]
Sriskandarajah, Sridevy [2 ]
Muller, Renate [1 ]
机构
[1] Univ Copenhagen, Dept Agr Sci, Fac Life Sci, DK-2630 Taastrup, Denmark
[2] Uppsala Bioctr, Dept Plant Biol & Forest Genet, SE-75007 Uppsala, Sweden
来源
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY | 2009年 / 84卷 / 02期
关键词
HAIRY-ROOT CULTURES; PLANT-REGENERATION; ESTABLISHMENT; GROWTH; INFECTION; ASSAYS;
D O I
10.1080/14620316.2009.11512505
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
Transgenic hairy roots were induced from leaf, petiole, and shoot explants of in vitro-raised plantlets of potted Hibiscus rosa-sinensis L. 'Cassiopeia Wind Yellow' plants after co-cultivation with three different wild-type strains of Agrobacterium rhizogenes. The highest infection rate (56.4%) was obtained in explants inoculated with A. rhizogenes strain ATCC43057, followed by ATCC15834 (44.0%), and NCPPB8196 (34.4%). The highest infection rate (60.5%) was obtained using leaf basal segments, followed by petioles (44.0%), leaf tips (40.0%), and shoots (20%). A total of 102 putative transgenic root lines were produced. The hairy root lines did not grow without applying 0.2 mg l(-1) indole-3-butryric acid (IBA) and 0.2 mg l(-1) alpha-naphthaleneacetic acid (NAA). The presence of T-DNA was confirmed in six hairy root lines, and no A. rhizogenes contamination was detected by polymerase chain reaction.
引用
收藏
页码:204 / 208
页数:5
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