Chromatophores efficiently promote light-driven ATP synthesis and DNA transcription inside hybrid multicompartment artificial cells

被引:49
作者
Altamura, Emiliano [1 ]
Albanese, Paola [1 ]
Marotta, Roberto [2 ]
Milano, Francesco [3 ]
Fiore, Michele [4 ]
Trotta, Massimo [5 ]
Stano, Pasquale [6 ]
Mavelli, Fabio [1 ,7 ]
机构
[1] Univ Bari Aldo Moro, Chem Dept, I-70125 Bari, Italy
[2] Italian Inst Technol, Electron Microscopy Facil, I-16163 Genoa, Italy
[3] CNR, Inst Sci Food Prod, I-73100 Lecce, Italy
[4] Univ Lyon Claude Bernard Lyon 1, Inst Chim & Biochim Mol & Supramol, F-69622 Villeurbanne, France
[5] CNR, IPCF, I-70125 Bari, Italy
[6] Univ Salento, Dept Biol & Environm Sci & Technol, I-73100 Lecce, Italy
[7] CNR, Inst Nanotechnol, I-70125 Bari, Italy
关键词
artificial protocells; artificial photosynthesis; bacterial chromatophores; light transduction; synthetic biology; PHOTOSYNTHETIC MEMBRANE; REACTION CENTERS; VESICLES; PROTEIN; COMPLEXES; SYNTHASE; EXPRESSION; ROTATION; ENERGY; MODEL;
D O I
10.1073/pnas.2012170118
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The construction of energetically autonomous artificial protocells is one of the most ambitious goals in bottom-up synthetic biology. Here, we show an efficient manner to build adenosine 5'-triphosphate (ATP) synthesizing hybrid multicompartment protocells. Bacterial chromatophores from Rhodobacter sphaeroides accomplish the photophosphorylation of adenosine 5'-diphosphate (ADP) to ATP, functioning as nanosized photosynthetic organellae when encapsulated inside artificial giant phospholipid vesicles (ATP production rate up to similar to 100 ATP.s(-1) per ATP synthase). The chromatophore morphology and the orientation of the photo-phosphorylation proteins were characterized by cryo-electron microscopy (cryo-EM) and time-resolved spectroscopy. The freshly synthesized ATP has been employed for sustaining the transcription of a DNA gene, following the RNA biosynthesis inside individual vesicles by confocal microscopy. The hybrid multicompartment approach here proposed is very promising for the construction of full-fledged artificial protocells because it relies on easy-to-obtain and ready-to-use chromatophores, paving the way for artificial simplified-autotroph protocells (ASAPs).
引用
收藏
页数:9
相关论文
共 60 条
[1]   Optimizing Enzymatic Photo-Redox Cycles by a Hybrid Protein Complex Chain [J].
Altamura, Emiliano ;
Albanese, Paola ;
Milano, Francesco ;
Giotta, Livia ;
Trotta, Massimo ;
Ferretta, Anna ;
Cocco, Tiziana ;
Mavelli, Fabio .
CHEMPHOTOCHEM, 2021, 5 (01) :26-31
[2]  
Altamura E, 2018, SYN BIOL, V3, DOI 10.1093/synbio/ysy011
[3]   First moves towards photoautotrophic synthetic cells: In vitro study of photosynthetic reaction centre and cytochrome bc1 complex interactions [J].
Altamura, Emiliano ;
Fiorentino, Rosa ;
Milano, Francesco ;
Trotta, Massimo ;
Palazzo, Gerardo ;
Stano, Pasquale ;
Mavelli, Fabio .
BIOPHYSICAL CHEMISTRY, 2017, 229 :46-56
[4]   Highly oriented photosynthetic reaction centers generate a proton gradient in synthetic protocells [J].
Altamura, Emiliano ;
Milano, Francesco ;
Tangorra, Roberto R. ;
Trotta, Massimo ;
Hassan Omar, Omar ;
Stano, Pasquale ;
Mavelli, Fabio .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (15) :3837-3842
[5]   Artificial photosynthetic cell producing energy for protein synthesis [J].
Berhanu, Samuel ;
Ueda, Takuya ;
Kuruma, Yutetsu .
NATURE COMMUNICATIONS, 2019, 10
[6]   Progress Toward Synthetic Cells [J].
Blain, J. Craig ;
Szostak, Jack W. .
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 83, 2014, 83 :615-640
[7]   Artificial Cells: Synthetic Compartments with Life-like Functionality and Adaptivity [J].
Buddingh, Bastiaan C. ;
van Hest, Jan C. M. .
ACCOUNTS OF CHEMICAL RESEARCH, 2017, 50 (04) :769-777
[8]   The lipidome of the photosynthetic bacterium Rhodobacter sphaeroides R26 is affected by cobalt and chromate ions stress [J].
Calvano, Cosima Damiana ;
Italiano, Francesca ;
Catucci, Lucia ;
Agostiano, Angela ;
Cataldi, Tommaso R. I. ;
Palmisano, Francesco ;
Trotta, Massimo .
BIOMETALS, 2014, 27 (01) :65-73
[9]   Integration of energy and electron transfer processes in the photosynthetic membrane of Rhodobacter sphaeroides [J].
Cartron, Michael L. ;
Olsen, John D. ;
Sener, Melih ;
Jackson, Philip J. ;
Brindley, Amanda A. ;
Qian, Pu ;
Dickman, Mark J. ;
Leggett, Graham J. ;
Schulten, Klaus ;
Hunter, C. Neil .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2014, 1837 (10) :1769-1780
[10]   Quantitative Analysis of the Lamellarity of Giant Liposomes Prepared by the Inverted Emulsion Method [J].
Chiba, Masataka ;
Miyazaki, Makito ;
Ishiwata, Shin'ichi .
BIOPHYSICAL JOURNAL, 2014, 107 (02) :346-354