Biofunctionalized Nanostructured Zirconia for Biomedical Application: A Smart Approach for Oral Cancer Detection

被引：117

作者：

Kumar, Suveen ^{[1
]}

Kumar, Saurabh ^{[1
]}

Tiwari, Sachchidanand ^{[1
]}

Srivastava, Saurabh ^{[1
]}

Srivastava, Manish ^{[2
]}

Yadav, Birendra Kumar ^{[3
]}

Kumar, Saroj ^{[1
]}

Thien Toan Tran ^{[4
]}

Dewan, Ajay Kumar ^{[3
]}

Mulchandani, Ashok ^{[4
]}

Sharma, Jai Gopal ^{[1
]}

Maji, Sagar ^{[1
]}

Malhotra, Bansi Dhar ^{[1
]}

机构：

[1] Delhi Technol Univ, Dept Biotechnol, Nanobioelect Lab, New Delhi 110042, India

[2] Univ Delhi, Dept Phys & Astrophys, New Delhi 110007, India

[3] Rajiv Gandhi Canc Inst & Res Ctr, New Delhi 110085, India

[4] Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92521 USA

来源：

ADVANCED SCIENCE | 2015年 / 2卷 / 08期

关键词：

ELECTROCHEMICAL IMMUNOSENSOR; BIOSENSOR; LABEL; NANOPARTICLES; SENSOR;

D O I：

10.1002/advs.201500048

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Results of the studies are reported relating to application of the silanized nanostructured zirconia, electrophoretically deposited onto indium tin oxide (ITO) coated glass for covalent immobilization of the monoclonal antibodies (anti-CYFRA-21-1). This biosensing platform has been utilized for a simple, efficient, noninvasive, and label-free detection of oral cancer via cyclic voltammetry technique. The results of electrochemical response studies conducted on bovine serum albumin (BSA)/anti-CYFRA-21-1/3-aminopropyl triethoxy silane (APTES)/ZrO2/ITO immunoelectrode reveal that this immunoelectrode can be used to measure CYFRA-21-1 (oral cancer biomarker) concentration in saliva samples, with a high sensitivity of 2.2 mA mL ng(-1), a linear detection range of 2-16 ng mL(-1), and stability of six weeks. The results of these studies have been validated via enzyme-linked immunosorbent assay.

引用

页数：9

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