RNAi in Budding Yeast

被引：405

作者：

Drinnenberg, Ines A. ^{[1
,2
]}

Weinberg, David E. ^{[1
,2
,3
]}

Xie, Kathleen T. ^{[1
,2
,3
]}

Mower, Jeffrey P. ^{[4
]}

Wolfe, Kenneth H. ^{[4
]}

Fink, Gerald R. ^{[1
,3
]}

Bartel, David P. ^{[1
,2
,3
]}

机构：

[1] Whitehead Inst Biomed Res, Cambridge, MA 02142 USA

[2] MIT, Howard Hughes Med Inst, Cambridge, MA 02139 USA

[3] MIT, Dept Biol, Cambridge, MA 02139 USA

[4] Univ Dublin Trinity Coll, Smurfit Inst Genet, Dublin 2, Ireland

来源：

SCIENCE | 2009年 / 326卷 / 5952期

基金：

爱尔兰科学基金会;

关键词：

RESOLUTION; EVOLUTION; RIBONUCLEASE; INITIATION; ELEMENTS;

D O I：

10.1126/science.1176945

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

RNA interference (RNAi), a gene-silencing pathway triggered by double-stranded RNA, is conserved in diverse eukaryotic species but has been lost in the model budding yeast Saccharomyces cerevisiae. Here, we show that RNAi is present in other budding yeast species, including Saccharomyces castellii and Candida albicans. These species use noncanonical Dicer proteins to generate small interfering RNAs, which mostly correspond to transposable elements and Y' subtelomeric repeats. In S. castellii, RNAi mutants are viable but have excess Y' messenger RNA levels. In S. cerevisiae, introducing Dicer and Argonaute of S. castellii restores RNAi, and the reconstituted pathway silences endogenous retrotransposons. These results identify a previously unknown class of Dicer proteins, bring the tool of RNAi to the study of budding yeasts, and bring the tools of budding yeast to the study of RNAi.

引用

页码：544 / 550

页数：7

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