Differentiation of Gastric Helicobacter Species Using MALDI-TOF Mass Spectrometry

被引：15

作者：

Berlamont, Helena ^{[1
]}

De Witte, Chloe ^{[1
]}

De Bruyckere, Sofie ^{[1
]}

Fox, James G. ^{[2
]}

Backert, Steffen ^{[3
]}

Smet, Annemieke ^{[4
]}

Boyen, Filip ^{[1
]}

Haesebrouck, Freddy ^{[1
]}

机构：

[1] Univ Ghent, Fac Vet Med, Dept Pathol Bacteriol & Poultry Dis, B-9820 Merelbeke, Belgium

[2] MIT, Div Comparat Med, Cambridge, MA 02139 USA

[3] Univ Erlangen Nurnberg, Dept Biol, Div Microbiol, D-91058 Erlangen, Germany

[4] Univ Antwerp, Fac Med & Hlth Sci, Lab Expt Med & Pediat, B-2610 Antwerp, Belgium

来源：

PATHOGENS | 2021年 / 10卷 / 03期

关键词：

gastric Helicobacter species; NHPH; MALDI-TOF MS; DESORPTION IONIZATION-TIME; ROUTINE IDENTIFICATION; RAPID IDENTIFICATION; CULTURE-CONDITIONS; BACTERIA; PATHOGENESIS; INFECTION; UREASE; CLASSIFICATION; PREVALENCE;

D O I：

10.3390/pathogens10030366

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Gastric helicobacters (Helicobacter (H.) pylori and non-H. pylori Helicobacter species (NHPHs)) colonize the stomach of humans and/or animals. Helicobacter species identification is essential since many of them are recognized as human and/or animal pathogens. Currently, Helicobacter species can only be differentiated using molecular methods. Differentiation between NHPHs using MALDI-TOF MS has not been described before, probably because these species are poorly represented in current MALDI-TOF MS databases. Therefore, we identified 93 gastric Helicobacter isolates of 10 different Helicobacter species using MALDI-TOF MS in order to establish a more elaborate Helicobacter reference database. While the MALDI Biotyper database was not able to correctly identify any of the isolates, the in-house database correctly identified all individual mass spectra and resulted in 82% correct species identification based on the two highest log score matches (with log scores >= 2). In addition, a dendrogram was constructed using all newly created main spectrum profiles. Nine main clusters were formed, with some phylogenetically closely related Helicobacter species clustering closely together and well-defined subclusters being observed in specific species. Current results suggest that MALDI-TOF MS allows rapid differentiation between gastric Helicobacter species, provided that an extensive database is at hand and variation due to growth conditions and agar-medium-related peaks are taken into account.

引用

页数：15

共 55 条

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