Protein versus DNA as a marker for peripheral blood mononuclear cell counting

被引：12
作者：
Jansen, Robert S. [1 ]
Rosing, Hilde [1 ]
Schellens, Jan H. M. [2 ,3 ]
Beijnen, Jos H. [1 ,3 ]
机构：
[1] Slotervaart Hospital, Netherlands Canc Inst, Dept Pharm & Pharmacol, NL-1066 EC Amsterdam, Netherlands
[2] Netherlands Canc Inst, Div Med Oncol, NL-1066 EC Amsterdam, Netherlands
[3] Univ Utrecht, Fac Sci, Dept Pharmaceut Sci, NL-3584 CA Utrecht, Netherlands
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2009年 / 395卷 / 03期
关键词：
Bioanalytical methods; Nucleic acids (DNA vertical bar RNA); Cell counting; Hoechst; 33258; CHROMATOGRAPHY/TANDEM MASS-SPECTROMETRY;
D O I：
10.1007/s00216-009-3022-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Quantitative analysis of intracellular analytes requires an accurate and precise assay not only for the quantitation of the analytes, but also for the quantitation of the number of cells in which they were determined. In this technical note we compare protein and DNA as markers for the number of peripheral blood mononuclear cells (PBMCs) isolated from whole blood. The protein content of samples was highly influenced by red blood cell contamination and was, therefore, a less suitable marker. The DNA-based method was unaffected by red blood cell contamination and was finally validated over a range from 10 x 10(6) to 300 x 10(6) PBMCs/mL.
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收藏
页码：863 / 867
页数：5
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