Selective probing of mRNA expression levels within a living cell

被引：29

作者：

Nawarathna, D. ^{[1
]}

Turan, T. ^{[2
]}

Wickramasinghe, H. Kumar ^{[1
,3
]}

机构：

[1] Univ Calif Irvine, Dept Elect & Comp Sci, Irvine, CA 92697 USA

[2] Univ Calif Irvine, Dept Pathol, Irvine, CA 92697 USA

[3] Univ Calif Irvine, Dept Biomed Engn, Irvine, CA 92697 USA

来源：

APPLIED PHYSICS LETTERS | 2009年 / 95卷 / 08期

关键词：

atomic force microscopy; biosensors; cellular biophysics; electrophoresis; macromolecules; molecular biophysics; organic compounds; REAL-TIME;

D O I：

10.1063/1.3213343

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We report on a selective and nondestructive measurement of mRNA (messenger ribonucleic acid) expression levels within a living cell. We first modify an atomic force microscope tip to create a tapered nanoscale coaxial cable. Application of an ac (alternating potential) between the inner and outer electrodes of this cable creates a dielectrophoretic force attracting mRNA molecules toward the tip-end which is pretreated with gene specific primers. We selectively extracted and analyzed both high (similar to 2500) and extremely low (110) copy number mRNA from a living cell mRNA in less than 10 s.

引用

页数：3

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