TiO2 nanotubes promote osteogenic differentiation of mesenchymal stem cells via regulation of lncRNA CCL3-AS

被引：12

作者：

Jin, Ziyang ^{[1
]}

Yan, Xufeng ^{[1
]}

Shen, Ke ^{[1
]}

Fang, Xingtang ^{[1
]}

Zhang, Chunlei ^{[1
]}

Ming, Qinglei ^{[1
]}

Lai, Min ^{[1
]}

Cai, Kaiyong ^{[2
]}

机构：

[1] Jiangsu Normal Univ, Sch Life Sci, Xuzhou 221116, Jiangsu, Peoples R China

[2] Chongqing Univ, Coll Bioengn, Minist Educ, Key Lab Biorheol Sci & Technol, Chongqing 400044, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2019年 / 181卷

关键词：

Mesenchymal stem cells; TiO2; nanotubes; LncRNA CCL3-AS; Osteogenic differentiation; FALSE DISCOVERY RATE; OSTEOBLAST DIFFERENTIATION; BONE-DISEASE; MECHANISMS; SURFACES; TRANSCRIPTION; INHIBITION; LANDSCAPE; ADHESION; GROWTH;

D O I：

10.1016/j.colsurfb.2019.05.041

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Titanium (Ti) is widely used as orthopedic implant materials. TiO2 nanotubes (TNTs) further improve the bioactivity of Ti, which enhance the osteogenic differentiation of mesenchymal stem cells (MSCs). However, the underlying mechanism is still unclear. In this study, we verified the response of MSCs on Ti and TNT substrates and explored the regulatory mechanism of long non-coding RNAs (lncRNAs). LncRNA and mRNA expression profiles were analyzed via RNA sequencing. Differential lncRNA and mRNA expression and predicted target genes of lncRNAs were performed by bioinformatics analysis. 1075 up-regulated and 1301 down-regulated genes, 26 up-regulated and 35 down-regulated lncRNAs were obtained according to the RNA-Seq. Expression of 8 lncRNAs were verified by qPCR, which was consistent with the sequencing data. To explore the function and target gene of lncRNA, lncRNA CCL3-AS and gene CCL3 were selected for further investigation. The fluorescence staining, alkaline phosphatase (ALP) activity and CCK-8 assay were performed. Besides, expressions of runt related transcription factor 2 (Runx2), collagen type I (Col I), osteopontin (OPN) were detected by qPCR and western blot. These results indicate that lncRNA CCL3-AS could inhibit the osteogenic differentiation and enhance cell viability of MSCs on the TNT substrates, which was dependent on the regulation of CCL3. This study supplied a comprehensive understanding for further study using lncRNA modulators to surface design of titanium for enhancing osseointegration.

引用

页码：416 / 425

页数：10

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