Micro-LC determination of swertiamarin in Swertia species and bacoside-A in Bacopa monnieri

被引：26

作者：

Bhandari, P. ^{[1
]}

Kumar, N. ^{[1
]}

Gupta, A. P. ^{[1
]}

Singh, B. ^{[1
]}

Kaul, V. K. ^{[1
]}

机构：

[1] Inst Himalayan Bioresource Technol, Nat Plant Prod Div, Palampur 176061, Himachal Prades, India

来源：

CHROMATOGRAPHIA | 2006年 / 64卷 / 9-10期

关键词：

column liquid chromatography; microanalytical technique; marker compounds; swertiamarin; bacoside-A;

D O I：

10.1365/s10337-006-0065-x

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A microanalytical HPLC technique has been developed for determination of swertiamarin in Swertia species and bacoside-A in Bacopa monnieri. Small quantities (>= 2 mg) of powdered plant material are required for analysis. The limits of detection (LOD) of swertiamarin and bacoside-A are 500 pg and 4,000 ng, respectively, in 2 mg plant material. Dried plant material from S. cordata and S. chirata contained 5.59 and 0.44% swertiamarin, respectively, and B. monnieri contained 0.12% bacoside-A. The simplicity, accuracy, and reproducibility of the method enable its use for estimation of marker compounds.

引用

页码：599 / 602

页数：4

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