Cyclo-Ketal Xanthene Dyes: A New Class of Near-Infrared Fluorophores for Super-Resolution Imaging of Live Cells

被引：43

作者：

Zhang, Xinfu ^{[1
]}

Chen, Lingcheng ^{[1
]}

Huang, Zhenlong ^{[1
]}

Ling, Ni ^{[1
]}

Xiao, Yi ^{[1
]}

机构：

[1] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2021年 / 27卷 / 11期

基金：

中国国家自然科学基金;

关键词：

element replacement; near-infrared fluorophore; super-resolution imaging; xanthene dyes; FLUORESCENT DYES; LONG-WAVELENGTH; DESIGN; RED; MICROSCOPY; RHODAMINE; OXYGEN; PROBE;

D O I：

10.1002/chem.202005296

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Newly emerging super-resolution imaging techniques provide opportunities for precise observations on cellular microstructures. However, they also impose severe demands on fluorophores. Here, we develop a new series of NIR xanthene dyes, named as KRhs, by replacing the 10-position O of rhodamines with a cyclo-ketal. KRhs display an intense NIR emission peak at 700 nm with fluorescence quantum yields up to 0.64. More importantly, they, without the aid of enhancing buffer, exhibit stochastic fluorescence off-on switches to support time-resolved localization of single fluorophore. KRhs are functionalized into KRh-MitoFix, KRh-Mem and KRh-Halo that demonstrate mitochondria, plasma membrane and fusion protein targeting ability, respectively. Consequently, these KRh probes demonstrate straightforward usage for super-resolution imaging of these targets in live cells. Therefore, KRhs merit future development for fluorescence labeling and super-resolution imaging in the NIR region.

引用

页码：3688 / 3693

页数：6