Estimation of minimum electron dose necessary to resolve molecular structure of deoxyribonucleic acid by phase transmission electron microscopy

被引：4

作者：

Nomaguchi, Tsunenori ^{[1
]}

Kimura, Yoshihide ^{[1
]}

Takai, Yoshizo ^{[1
]}

机构：

[1] Osaka Univ, Dept Mat & Life Sci, Grad Sch Engn, Suita, Osaka 5650871, Japan

来源：

APPLIED PHYSICS LETTERS | 2006年 / 89卷 / 23期

基金：

日本科学技术振兴机构;

关键词：

D O I：

10.1063/1.2402230

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

The minimum electron dose that is necessary to resolve the molecular structure of deoxyribonucleic acid (DNA) was estimated based on experimental measurements of information limits and simulated DNA images, considering conditions of a low electron dose. From these results, a dose of similar to 400 e/A(2) was found to be necessary to achieve observation of DNA on a molecular scale under the present experimental setup. A DNA molecule was observed by a phase reconstruction method using through-focus images under the limited electron dose. In the reconstructed images, the helical structure and the intervals of the base pairs of DNA were partially resolved. (c) 2006 American Institute of Physics.

引用

页数：3

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