Advances in capillary electrophoresis-mass spectrometry for cell analysis

被引：35
作者：
Zhou, Wei [1 ,2 ,3 ]
Zhang, Baofang [1 ,2 ]
Liu, Yikun [1 ,2 ]
Wang, Chenlu [1 ,2 ]
Sun, Wenqi [1 ,2 ]
Li, Wen [1 ,2 ]
Chen, Zilin [1 ,2 ,3 ]
机构：
[1] Minist Educ, Key Lab Combinatorial Biosynth & Drug Discovery, Wuhan 430071, Hubei, Peoples R China
[2] Wuhan Univ, Sch Pharmaceut Sci, Wuhan 430071, Hubei, Peoples R China
[3] Chinese Acad Sci, State Key Lab Transducer Technol, Beijing 10080, Peoples R China
来源：
TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2019年 / 117卷 / 316-330期
基金：
中国国家自然科学基金;
关键词：
Capillary electrophoresis; Mass spectrometry; Cell analysis; Interface; Biomacromolecule analysis; Cell metabolism; Single cell analysis; SHEATH-FLOW INTERFACE; LIQUID-LIQUID MICROEXTRACTION; HIGH PEAK-CAPACITY; MONOCLONAL-ANTIBODY; METABOLOMIC ANALYSIS; TOP-DOWN; TRANSIENT ISOTACHOPHORESIS; PEPTIDE IDENTIFICATIONS; IMPROVED SENSITIVITY; ESTROGENIC COMPOUNDS;
D O I：
10.1016/j.trac.2019.05.011
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Mass spectrometry has become a powerful tool in the field of bioanalysis. The combination of capillary electrophoresis with mass spectrometry (CE-MS) offers fast and high-resolution separation, sensitive and selective detection with small sample volumes, which is suitable for cell analysis. Here, we review the recent technical development of CE, MS and hyphenated strategies as well as their potential in cell analysis. In particular, we discuss the multiple MS compatible CE modes including capillary zone electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic capillary chromatography and capillary electrochromatography for achieving better separation efficiency and selectivity before MS detection. For enhancing the sensitivity of CE-MS, several online preconcentration methods including dynamic pH junction, transient isotachophoresis and solid phase extraction are also reviewed and discussed. We also summarize the existing applications of CE-MS in cell analysis including cell biomacromolecule analysis, cell metabolism and single cell analysis. (C) 2019 Elsevier B.V. All rights reserved.
引用
收藏
页码：316 / 330
页数：15
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