Urea inhibition of renal (NA++K+)ATPase activity is reversed by cAMP

被引：5

作者：

Silva, IV ^{[1
]}

Caruso-Neves, C ^{[1
]}

Azeredo, IM ^{[1
]}

Carvalho, TLG ^{[1
]}

Lara, LS ^{[1
]}

de Mello, MC ^{[1
]}

Lopes, AG ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, CCS, BR-219900 Rio De Janeiro, Brazil

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2002年 / 406卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

(NA(+) + K+)ATPase; urea; cAMP; signal transduction; phosphorylation;

D O I：

10.1016/S0003-9861(02)00405-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present work we studied the modulation of the effect of urea on the renal (Na+ + K+)ATPase by cAMP. We observed that urea inhibits the (NA(+) + K+)ATPase activity in a dose-dependent manner, reaching 60% of inhibition at the concentration of 1 M. This effect was completely reversed by dibutyryl-cAMP (dBcAMP) at 5 x 10(-4) M. The effect of dBcAMP was mimicked by 50 units of the catalytic subunit of protein kinase A and completely abolished by 5 x 10(-7) M H89, an inhibitor of protein kinase A. Addition of 1 M urea decreases basal phosphorylation of the immunoprecipitated (NA(+) + K+)ATPase in 50%, with this effect completely reversed by 5 x 10(-4) M dBcAMP. Furthermore, 5 x 10(-4) M dBcAMP by itself induced (NA(+) + K+) ATPase phosphorylation. Taken together these data indicate that cAMP could be, in addition to the organic solutes already known, an important physiological modulator of the deleterious effect of urea on enzyme activity. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：183 / 189

页数：7

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