Morphology of Testes from Transgenic Rabbits: Histological and Ultrastructural Aspects

被引:2
作者
Chrenek, P. [1 ,2 ]
Makarevich, A. V. [1 ]
Kubovicova, E. [1 ]
Pivko, J. [1 ]
机构
[1] Anim Prod Res Ctr, Luzianky 95141, Slovakia
[2] Slovak Univ Agr, Fac Food Sci & Biotechnol, Nitra, Slovakia
关键词
CONTRACEPTIVE DEVELOPMENT; SERTOLI-SERTOLI; CELL; SPERMATOGENESIS; SPERMATOZOA; JUNCTIONS; RAT;
D O I
10.1111/j.1439-0264.2009.00970.x
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
The aim of this study was to compare morphological characteristics of testes from transgenic (the WAP-hFVIII gene) and non-transgenic rabbits with emphasis on the histological and ultrastructural aspects. Samples of testes from both groups were fixed and embedded into Durcupan ACM for transmission electron microscopy. For histological analysis, semi-thin toluidine blue-stained sections were evaluated under a Jenaval light microscope. Male fertility was tested based on egg fecundity and blastocyst yield; transgene transmission was proved using PCR assay. Spermatogenesis in rabbit testes had not been destroyed both in transgenic and non-transgenic rabbits. No significant differences were found in the occurrence of individual cell organelles of the Sertoli cells in transgenic and non-transgenic rabbits. The ultrastructure of Leydig cells in testes of transgenic and non-transgenic rabbits was rather similar. No differences in the occurrence of individual organelles of Leydig cells between transgenic and non-transgenic males were found. These results were in concert with fertilizing capacity of transgenic spermatozoa. The presented status of organelles in this study indicates functional activity of the analysed cells.
引用
收藏
页码:27 / 33
页数:7
相关论文
共 21 条
[1]  
CHEVILLE NF, 1994, ULTRASTRUCT PATHOL, P502
[2]   Increased transgene integration efficiency upon microinjection of DNA into both pronuclei of rabbit embryos [J].
Chrenek, P ;
Vasicek, D ;
Makarevich, A ;
Jurcik, R ;
Suvegova, K ;
Parkanyi, V ;
Bauer, M ;
Rafay, J ;
Batorova, A ;
Paleyanda, RK .
TRANSGENIC RESEARCH, 2005, 14 (04) :417-428
[3]  
Chrenek P, 2005, B VET I PULAWY, V49, P307
[4]  
Chrenek P, 2007, SLOVAK J ANIM SCI, V40, P113
[5]  
CHRENEK P, 2006, SLOVAK J ANIM SCI, V39, P131
[6]   Effect of transgenesis on reproductive traits of rabbit males [J].
Chrenek, Peter ;
Trandzik, Jozef ;
Massanyi, Peter ;
Makarevich, Alexander ;
Lukac, Norbert ;
Peskovicova, Dana ;
Paleyanda, Rekha .
ANIMAL REPRODUCTION SCIENCE, 2007, 99 (1-2) :127-134
[7]  
Christensen A.K., 1975, HDB PHYSL, P57
[8]  
Christensen A.K., 1969, GONADS, P415
[9]  
DEKRETSER DM, 1980, TESTICULAR DEV STRUC, P107
[10]  
Fawcett D.W., 1975, HDB PHYSL, V5, P21