Analysis of murine S-glutathionyl hemoglobins and beta globin haplotype by dynamic capillary isoelectric focusing

被引：5
作者：
Hempe, James M. [1 ,2 ]
McGehee, Amanda M. [1 ]
Stoyanov, Alexandre V. [1 ]
机构：
[1] Childrens Hosp, Res Inst Children, New Orleans, LA 70118 USA
[2] Louisiana State Univ, Hlth Sci Ctr, Dept Pediat, New Orleans, LA USA
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 28期
关键词：
Hemoglobin; S-glutathionyl hemoglobin; Glutathione; Mouse models; Alpha globin; Beta globin; MOUSE; METABOLISM;
D O I：
10.1016/j.jchromb.2009.06.013
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Genetic variation in the number and reactivity of beta globin sulfhydryl groups causes variation in erythrocyte redox status in mouse populations. These experiments demonstrate the use of capillary isoelectric focusing for measuring endogenous S-glutathionyl hemoglobin and identifying mouse beta globin (Hbb) haplotype in inbred and outbred mouse strains with mono-cysteinyl or di-cysteinyl beta globins. Hbb haplotype can be readily determined in all strains based on characteristic differences in peak profiles or on peak mobility shift induced by thiol exchange with glutathione disulfide in vitro. This method could prove useful for in vivo study of factors that influence thiol protein modification. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：3462 / 3466
页数：5
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