MHC-dependent cytolysis of autologous tumor cells by lymphocytes infiltrating urothelial carcinomas

被引:0
作者
Housseau, F
Zeliszewski, D
Roy, M
Paradis, V
Richon, S
Ricour, A
Bougaran, J
Prapotnich, D
Vallancien, G
Benoit, G
Desportes, L
Bedossa, P
Hercend, T
Bidart, JM
Bellet, D
机构
[1] UNIV PARIS 05,URA 1484 CNRS,LAB TUMOR IMMUNOL,PARIS,FRANCE
[2] HOP BICETRE,DEPT PATHOL,LE KREMLIN BICETR,FRANCE
[3] INST MUTUALISTE MONSOURIS,DEPT PATHOL,PARIS,FRANCE
[4] INST MUTUALISTE MONSOURIS,DEPT UROL,PARIS,FRANCE
[5] HOP BICETRE,DEPT UROL,LE KREMLIN BICETR,FRANCE
[6] INSERM,U267,LAB IMMUNOGEN ALLOGRAFT,VILLEJUIF,FRANCE
[7] INST GUSTAVE ROUSSY,DEPT ONCOL BIOL,VILLEJUIF,FRANCE
关键词
D O I
10.1002/(SICI)1097-0215(19970516)71:4<585::AID-IJC13>3.0.CO;2-B
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Tumor-infiltrating lymphocytes (TIL) were grown from 23 urothelial carcinomas. Phenotyping analysis showed that the TIL cultures were mainly CD3(+). Although CD4(+) and CD8(+) T-cell sub-sets were grown in culture, CD4(+) T-cell sub-sets predominated over CD8(+) T cells, Immunohistochemical studies performed on 5 tumor specimens confirmed this observation, and indicated that CD4(+) T cells surrounded the tumor islets, whereas CD8(+) T lymphocytes were localized among the tumor cells. Five short-term carcinoma cell lines established from these urothelial tumors were used as target cells in cytolysis assays in order to investigate the functional anti-tumor activity of autologous TIL, TIL from 4/5 tumors were lytic and 3 TIL lines displayed MHC-class-l-dependent cytotoxicity directed against autologous tumor cells. CD4(+) T-cell-depletion experiments performed on TIL line 07 confirmed that CD8(+) MHC-class-l-dependent CTL were the predominant effecters. Finally, experiments performed on 6 allogeneic urothelial-cancer cell lines matched for HLA-class-I molecules showed that TIL07 exhibited selective lytic activity toward tumor 07, These data indicate that CD8(+) MHC-class-l-dependent CTL present in urothelial carcinomas are functional and may participate in the anti-tumor immune response. (C) 1997 Wiley-Liss, Inc.
引用
收藏
页码:585 / 594
页数:10
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