Changes in antibiotic resistance genes and mobile genetic elements during cattle manure composting after inoculation with Bacillus subtilis

被引：99

作者：

Duan, Manli ^{[1
]}

Zhang, Yuhua ^{[1
]}

Zhou, Beibei ^{[1
]}

Wang, Quanjiu ^{[1
]}

Gu, Jie ^{[2
]}

Liu, Guohuan ^{[1
]}

Qin, Zhenlun ^{[1
]}

Li, Zhijian ^{[1
]}

机构：

[1] Xian Univ Technol, State Key Lab Ecohydraul Engn Arid Area, Xian 710048, Shaanxi, Peoples R China

[2] Northwest A&F Univ, Coll Nat Resources & Environm, Yangling 712100, Shaanxi, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2019年 / 292卷

基金：

中国博士后科学基金; 美国国家科学基金会;

关键词：

Antibiotic resistance gene; Bacillus subtilis; Bacterial community; Composting; intI1; WATER TREATMENT PLANTS; BACTERIAL COMMUNITIES; ANTIMICROBIAL RESISTANCE; EMERGING CONTAMINANTS; ANAEROBIC-DIGESTION; SWINE-MANURE; ABUNDANCE; SLUDGE; SOIL; ASSOCIATION;

D O I：

10.1016/j.biortech.2019.122011

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

This study explored the effects of Bacillus subtilis at four levels (0, 0.5%, 1%, and 2% w/w compost) on the variations in ARG5, mobile genetic elements (MGE5), and the bacterial community during composting. The composting process had a greater impact on ARG5 than Bacillus subtilis. The main ARG detected was sul1. The addition of Bacillus subtilis at 0.5% reduced the relative abundances of ARG5, MGE5, and human pathogenic bacteria (by 2-3 logs) in the mature products. Network and redundancy analyses suggested that intI1, Firmicutes, and pH were mainly responsible for the changes in ARG5, thus controlling these factors might help to inhibit the spread of ARGs.

引用

页数：9

共 47 条

[1]

Afema JA, 2018, APPL ENVIRON MICROB, V84, DOI [10.1128/AEM.02430-17, 10.1128/aem.02430-17]

[2] Distinct effects of struvite and biochar amendment on the class 1 integron antibiotic resistance gene cassettes in phyllosphere and rhizosphere [J].

An, Xin-Li ;

Chen, Qing-Lin ;

Zhu, Dong ;

Su, Jian-Qiang .

SCIENCE OF THE TOTAL ENVIRONMENT, 2018, 631-632 :668-676

[3] Abundance of antibiotic resistance genes in environmental bacteriophages [J].

Anand, Taruna ;

Bera, Bidhan Ch. ;

Vaid, Rajesh K. ;

Barua, Sanjay ;

Riyesh, Thachamvally ;

Virmani, Nitin ;

Hussain, Mubarik ;

Singh, Raj K. ;

Tripathi, Bhupendra N. .

JOURNAL OF GENERAL VIROLOGY, 2016, 97 :3458-3466

[4] Composting of animal manures and chemical criteria for compost maturity assessment. A review [J].

Bernal, M. P. ;

Alburquerque, J. A. ;

Moral, R. .

BIORESOURCE TECHNOLOGY, 2009, 100 (22) :5444-5453

[5] Detecting Human Bacterial Pathogens in Wastewater Treatment Plants by a High-Throughput Shotgun Sequencing Technique [J].

Cai, Lin ;

Zhang, Tong .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2013, 47 (10) :5433-5441

[6] Do manure-borne or indigenous soil microorganisms influence the spread of antibiotic resistance genes in manured soil? [J].

Chen, Qing-Lin ;

An, Xin-Li ;

Li, Hu ;

Zhu, Yong-Guan ;

Su, Jian-Qiang. ;

Cui, Li .

SOIL BIOLOGY & BIOCHEMISTRY, 2017, 114 :229-237

[7] CONSIDERING PHARMACOKINETIC AND MECHANISTIC INFORMATION IN CANCER RISK ASSESSMENTS FOR ENVIRONMENTAL CONTAMINANTS - EXAMPLES WITH VINYL-CHLORIDE AND TRICHLOROETHYLENE [J].

CLEWELL, HJ ;

GENTRY, PR ;

GEARHART, JM ;

ALLEN, BC ;

ANDERSEN, ME .

CHEMOSPHERE, 1995, 31 (01) :2561-2578

[8]

Dajana K. G., 2019, ARAB J SCI ENG, V1, P1, DOI [10.1007/s13369-018-03692-8, DOI 10.1007/S13369-018-03692-8]

[9] Effects of genetically modified cotton stalks on antibiotic resistance genes, intI1, and intI2 during pig manure composting [J].

Duan, Manli ;

Gu, Jie ;

Wang, Xiaojuan ;

Li, Yang ;

Zhang, Sheqi ;

Zhang, Ranran .

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2018, 147 :637-642

[10] Dissemination of antibiotic resistance genes and human pathogenic bacteria from a pig feedlot to the surrounding stream and agricultural soils [J].

Fang, Hua ;

Han, Lingxi ;

Zhang, Houpu ;

Long, Zhengnan ;

Cai, Lin ;

Yu, Yunlong .

JOURNAL OF HAZARDOUS MATERIALS, 2018, 357 :53-62

← 1 2 3 4 5 →