In Situ Loading of Basic Fibroblast Growth Factor Within Porous Silica Nanoparticles for a Prolonged Release

被引：38

作者：

Zhang, Jin ^{[1
]}

Postovit, Lynne-Marie ^{[2
]}

Wang, Dashan ^{[3
]}

Gardiner, Richard B. ^{[4
]}

Harris, Richard ^{[4
]}

Abdul, Mumin Md ^{[1
]}

Thomas, Anu Alice ^{[1
]}

机构：

[1] Univ Western Ontario, Dept Chem & Biochem Engn, London, ON N6A 5B9, Canada

[2] Univ Western Ontario, Schulich Sch Med & Dent, Dept Anat & Cell Biol, London, ON N6A 5C1, Canada

[3] Natl Res Council Canada, Inst Chem Proc & Environm Technol, Ottawa, ON K1A 0R6, Canada

[4] Univ Western Ontario, Dept Biol, London, ON N6A 5B7, Canada

来源：

NANOSCALE RESEARCH LETTERS | 2009年 / 4卷 / 11期

基金：

加拿大自然科学与工程研究理事会;

关键词：

In situ loading method; Protein release; Nanoparticles; MESOPOROUS SILICA; DELIVERY; OPTIMIZATION; REGENERATION; EXPRESSION; DESIGN;

D O I：

10.1007/s11671-009-9395-6

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Basic fibroblast growth factor (bFGF), a protein, plays a key role in wound healing and blood vessel regeneration. However, bFGF is easily degraded in biologic systems. Mesoporous silica nanoparticles (MSNs) with well-tailored porous structure have been used for hosting guest molecules for drug delivery. Here, we report an in situ route to load bFGF in MSNs for a prolonged release. The average diameter (d) of bFGF-loaded MSNs is 57 +/- 8 nm produced by a water-in-oil microemulsion method. The in vitro releasing profile of bFGF from MSNs in phosphate buffer saline has been monitored for 20 days through a colorimetric enzyme linked immunosorbent assay. The loading efficiency of bFGF in MSNs is estimated at 72.5 +/- 3%. In addition, the cytotoxicity test indicates that the MSNs are not toxic, even at a concentration of 50 mu g/mL. It is expected that the in situ loading method makes the MSNs a new delivery system to deliver protein drugs, e. g. growth factors, to help blood vessel regeneration and potentiate greater angiogenesis.

引用

页码：1297 / 1302

页数：6

共 27 条

[1] Optimization of dye-doped silica nanoparticles prepared using a reverse microemulsion method [J].

Bagwe, RP ;

Yang, CY ;

Hilliard, LR ;

Tan, WH .

LANGMUIR, 2004, 20 (19) :8336-8342

[2]

Bharali DJ, 2005, P NATL ACAD SCI USA, V102, P11539, DOI 10.1073/pnas.0504926102

[3] CHARACTERIZATION OF BIOLOGIC PROPERTIES OF WOUND FLUID COLLECTED DURING EARLY STAGES OF WOUND-HEALING [J].

CHEN, WYJ ;

ROGERS, AA ;

LYDON, MJ .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1992, 99 (05) :559-564

[4] G-CSF loaded biodegradable PLGA nanoparticles prepared by a single oil-in-water emulsion method [J].

Choi, SH ;

Park, TG .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2006, 311 (1-2) :223-228

[5] HEPARIN-FIBROBLAST GROWTH FACTOR-FIBRIN COMPLEX - IN-VITRO AND IN-VIVO APPLICATIONS TO COLLAGEN-BASED MATERIALS [J].

DEBLOIS, C ;

COTE, MF ;

DOILLON, CJ .

BIOMATERIALS, 1994, 15 (09) :665-672

[6] CONTROLLED AND MODULATED RELEASE OF BASIC FIBROBLAST GROWTH-FACTOR [J].

EDELMAN, ER ;

MATHIOWITZ, E ;

LANGER, R ;

KLAGSBRUN, M .

BIOMATERIALS, 1991, 12 (07) :619-626

[7] HEPARIN PROTECTS BASIC AND ACIDIC FGF FROM INACTIVATION [J].

GOSPODAROWICZ, D ;

CHENG, J .

JOURNAL OF CELLULAR PHYSIOLOGY, 1986, 128 (03) :475-484

[8] Active growth factor delivery from poly(D,L-lactide-co-glycolide) foams prepared in supercritical CO2 [J].

Hile, DD ;

Amirpour, ML ;

Akgerman, A ;

Pishko, MV .

JOURNAL OF CONTROLLED RELEASE, 2000, 66 (2-3) :177-185

[9] Healing of full-thickness defects of the articular cartilage in rabbits using fibroblast growth factor-2 and a fibrin sealant [J].

Ishii, I. ;

Mizuta, H. ;

Sei, A. ;

Hirose, J. ;

Kudo, S. ;

Hiraki, Y. .

JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME, 2007, 89B (05) :693-700

[10]

LIU LS, 1998, P 25 INT S CONTR REL, V25, P996

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