Genetic diversity and population structure of natural populations of the endangered grass Paspalum rawitscheri (Parodi) Chase ex GH Rua & Valls (Paspaleae, Poaceae)

被引:2
作者
de Freitas, Jessica Mena Barreto [1 ]
Essi, Liliana [1 ]
Tedesco, Solange Bosio [1 ]
机构
[1] Univ Fed Santa Maria, Dept Biol, Santa Maria, RS, Brazil
关键词
Brazilian grasslands; Conservation; ISSR; Population structure; GENUS PASPALUM; ISSR MARKERS; RAPD;
D O I
10.1007/s40415-021-00699-8
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The objective of this study was to evaluate the genetic diversity and population structure of natural subpopulations of Paspalum rawitscheri (Parodi) Chase ex G.H. Rua & Valls using inter-simple sequence repeat (ISSR) molecular markers. Four natural subpopulations from State of Rio Grande do Sul, Brazil, were evaluated, using six ISSR primers. A total of 131 fragments were amplified, 34 of which were polymorphic. The genetic diversity (h) for the subpopulations was considered low, with values ranging from 0.130 to 0.217. The Vacaria subpopulation presented the lowest diversity among populations. The Shannon (I) diversity indices were also low, with values ranging from 0.187 to 0.334, with the Vacaria subpopulation also presenting the lowest values. The molecular variance (AMOVA) analysis showed, in general, the highest variation occurred within subpopulations rather than between subpopulations. The Bayesian Inference analysis separated the individuals into four genetic clusters, mixing individuals from different municipalities in the clusters. The results indicate that the species has low genetic diversity and low population structure, requiring conservation plans. Ex situ conservation becomes a necessary effort, since populations of this species are found in grasslands remnants affected by fires and implantation of crops, and is not present in any protected area of the Brazilian Conservation Units system.
引用
收藏
页码:401 / 409
页数:9
相关论文
共 42 条
  • [1] Alam A, 2008, AFR J BIOTECHNOL, V7, P1028
  • [2] [Anonymous], 2005, Monogr. Syst. Bot. Missouri Bot. Gard.
  • [3] [Anonymous], POACEAE FLORA BRASIL
  • [4] [Anonymous], 2013, LIVRO VERMELHO FLORA
  • [5] Preliminary characterization and selection of germoplasm of genus Paspalum for forage production
    Batista, LAR
    Godoy, R
    [J]. REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE, 2000, 29 (01): : 23 - 32
  • [6] Brasil, 2014, DIARIO OFICIAL
  • [7] Genetic diversity of the complex Paspalum notatum Flugge (Paniceae: Panicoideae)
    Cidade, Fernanda Witt
    Dall'Agnol, Miguel
    Bered, Fernanda
    de Souza-Chies, Tatiana Teixeira
    [J]. GENETIC RESOURCES AND CROP EVOLUTION, 2008, 55 (02) : 235 - 246
  • [8] Denham Silvia S., 2007, Aliso, V23, P545
  • [9] Doyle JJ., 1987, PHYTOCHEMISTRY B, V19, P11, DOI [DOI 10.2307/4119796, DOI 10.1016/0031-9422(80)85004-7]
  • [10] STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method
    Earl, Dent A.
    vonHoldt, Bridgett M.
    [J]. CONSERVATION GENETICS RESOURCES, 2012, 4 (02) : 359 - 361