Roles of calcium-dependent protein kinases mediated reactive oxygen species homeostasis in inducing resistance of apples by acibenzolar-S-methyl

被引：13
作者：
Hou, Jiabao [1 ,2 ]
Li, Canying [1 ,2 ]
Cheng, Yuan [1 ,2 ]
Jiang, Chaonan [1 ,2 ]
Li, Yihan [1 ,2 ]
Ge, Yonghong [1 ,2 ]
Li, Jianrong [1 ,2 ]
机构：
[1] Bohai Univ, Coll Food Sci & Technol, Jinzhou 121013, Peoples R China
[2] Natl & Local Joint Engn Res Ctr Storage Proc & Sa, Jinzhou 121013, Peoples R China
来源：
FOOD CHEMISTRY | 2021年 / 346卷
基金：
中国国家自然科学基金;
关键词：
Malus domestica; Calcium-dependent protein kinases; Reactive oxygen species; Induced resistance; Acibenzolar-S-methyl; DISEASE RESISTANCE; FRUIT; METABOLISM; RESPONSES; GENES; EXPRESSION; INDUCTION; INFECTION; EVENTS; SIGNAL;
D O I：
10.1016/j.foodchem.2020.128881
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
This study was carried out to investigate the effect of acibenzolar-S-methyl (ASM) and ethylenebis (oxyethylenenitrilo) tetraacetic acid (EGTA) treatments on calcium-dependent protein kinases (CDPKs) and reactive oxygen species (ROS) metabolism in apples. Postharvest ASM treatment increased H2O2 content, reduced glutathione and ascorbic acid contents, and NADPH oxidase, peroxidase, ascorbate peroxidase, superoxide dismutase and glutathione reductase activities and retarded catalase activity and MdCAT expression in apples. ASM treatment enhanced MdSOD, MdPOD, MdAPX, MdGR, MdCDPK1, MdCDPK4, MdCDPK5, MdCDPK7, and MdCDPK21 expressions in apples. However, EGTA + ASM treatments suppressed H2O2, glutathione and ascorbic acid contents, NADPH oxidase, peroxidase, superoxide dismutase, ascorbate peroxidase and glutathione reductase activities. EGTA + ASM treatments suppressed the selected genes expressions in ROS metabolism and CDPKs, but up-regulated MdCAT expression in apples. These findings suggest that CDPKs play a vital role in regulating ROS metabolism and involve in inducing resistance in apples by ASM.
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页数：9
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