RT-PCR for Detecting ALK Translocations in Cytology Samples from Lung Cancer Patients

被引：8

作者：

Nakamichi, Shinji ^{[1
]}

Seike, Masahiro ^{[1
]}

Miyanaga, Akihiko ^{[1
]}

Chiba, Mika ^{[1
]}

Matsuda, Kuniko ^{[1
]}

Kobayashi, Kenichi ^{[1
]}

Takahashi, Akiko ^{[1
]}

Takeuchi, Susumu ^{[1
]}

Minegishi, Yuji ^{[1
]}

Kubota, Kaoru ^{[1
]}

Gemma, Akihiko ^{[1
]}

机构：

[1] Nippon Med Sch, Grad Sch Med, Dept Pulm Med & Oncol, Tokyo, Japan

来源：

ANTICANCER RESEARCH | 2017年 / 37卷 / 06期

关键词：

ALK; RT-PCR; FISH; immunochemistry; cytology samples; EML4-ALK FUSION; TYROSINE KINASE; FISH; CRIZOTINIB; IMMUNOHISTOCHEMISTRY; CHEMOTHERAPY; IHC;

D O I：

10.21873/anticanres.11696

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background/Aim: We evaluated the usefulness of reverse transcription-polymerase chain reaction (RT-PCR) for detecting anaplastic lymphoma kinase (ALK) translocations using cytology samples from lung cancer patients. Materials and Methods: We analyzed ALK translocations by RT-PCR in cytology samples from lung cancer patients diagnosed at the Nippon Medical School Hospital between 2013 and 2015. Immunochemistry (IHC) and break-apart fluorescence in situ hybridization (FISH) were also performed on available tissue samples. Results: A total of 155 cytology samples were analyzed in our study. We obtained 115 (68%) samples from bronchial lavage. We were able to determine 153 (99%) results by RT-PCR with 4 (3%) positive samples. The four samples positive by RT-PCR were also positive by IHC and FISH performed on the tissue samples collected simultaneously. Conclusion: RT-PCR is a suitable method for detecting ALK translocations using cytology samples from patients with primary lung cancer, especially when tissue samples are not available.

引用

页码：3295 / 3299

页数：5

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