Morphometric and Biochemical Changes in Agave americana L. Plantlets Induced By Ethyl Methanesulfonate

被引：8

作者：

Reyes-Zambrano, S. J. ^{[1
]}

Ramirez-Merchant, M. L. ^{[1
]}

Arias-Castro, C. ^{[2
]}

Rodriguez-Mendiola, M. A. ^{[2
]}

Lecona-Guzman, C. A. ^{[1
]}

Ruiz-Valdiviezo, V. M. ^{[1
]}

Gonzalez-Mendoza, D. ^{[3
]}

Gutierrez-Miceli, F. A. ^{[1
]}

机构：

[1] Tecnol Nacl Mexico, Inst Tecnol Tuxtla Gutierrez, Lab Biotecnol Vegetal, Carretera Panamer Km 1080, Tuxtla Gutierrez 29050, Chiapas, Mexico

[2] Tecnol Nacl Mexico, DEPI Inst Tecnol Tlajomulco, Labs Anal Instrumental Bioquim, Tlajomulco De Zuniga 45640, Mexico

[3] UABC, ICA, Carretera Delta S-N, Ejido Nuevo Leon 21705, Baja California, Mexico

来源：

PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY | 2019年 / 88卷 / 03期

关键词：

Fructans; callus; mutagenesis; flavonoids; anthocyanins; phenylalanine ammonia lyase activity; IN-VITRO MUTAGENESIS; GENETIC-VARIABILITY; CULTIVAR; TOBACCO; GROWTH; GENERATION; INDUCTION; CULTURE;

D O I：

10.32604/phyton.2019.06504

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A. americana L. is a crop with very little genetic variability. In order to evaluate the effect of ethyl methanesulfonate (EMS) to induce variability in in vitro plantlets of A. americana, different explants (meristems, leaves and roots) were evaluated for the production of callus. MS medium supplemented with ANA (2.68 mu M) and BAP (2.68 mu M) was used. Callus obtained from apical meristem were treated with 15 mM EMS for two hours after which shoot formation was induced using 2,4-D (0.11 mu M) and BAP (44 mu M). The EMS induced variations in the morphometric and morphological parameters of the plantlets obtained, with 60% of the plantlets presenting differences such as dwarfism and different leaf forms, without the presence of spines, as well as an increase in fructan content of 30% with respect to the control plantlets. PAL was increased and this activity is related with higher anthocyanins concentration in A. americana L. plantlets.

引用

页码：277 / 284

页数：8

共 36 条

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