Effects of tachyplesin on proliferation and differentiation of human hepatocellular carcinoma SMMC-7721 cells

被引:31
作者
Ouyang, GL
Li, QF [1 ]
Peng, XX
Liu, QR
Hong, SG
机构
[1] Xiamen Univ, Sch Life Sci, Key Lab China Educ Minist Cell Biol & Tumor Cell, Xiamen 361005, Fujian Province, Peoples R China
[2] Xiamen Univ, Sch Life Sci, Cell Biol Lab, Xiamen 361005, Fujian Province, Peoples R China
关键词
D O I
10.3748/wjg.v8.i6.1053
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
AIM: To investigate the antitumor activities of tachyplesin on human hepatocellular carcinoma (HCC) cells. METHODS: Tachyplesin, isolated from acid extracts of Chinese horseshoe crab ( Tachypleus tridentatus) hemocytes, was used to treat the human HCC cell line SMMC-7721. Effects of tachyplesin on the proliferation of SMMC-7721 cells were measured with trypan blue dye exclusion test and HE staining. The morphology and ultrastructure of the cells were examined by light microscopy and transmission electron microscopy, respectively. The activities of gamma-glutamyltransferase (gamma-GT) and tyrosine aminotransferase (TAT) were assayed with biochemical methods. The levels of alpha fetoprotein (alpha-FP), proliferating cell nuclear antigen (PCNA), p21(WAF1/CIP1) and c-myc were examined by immunocytochemistry. RESULTS: After treatment with tachyplesin 3.0 mg/L, the proliferation of SMMC-7721 cells was inhibited significantly, with the cell growth inhibitory rate amounted to 55.57% and the maximum cell mitotic index declined by 43.68%. The morphology and ultrastructure underwent restorational alteration. The activity of gamma-GT declined while TAT activity increased obviously, and the levels of alpha-FP and PCNA decreased. Moreover, the expression of p21(WAF1/CIP1) protein was up-regulated and that of c-myc protein was down-regulated. CONCLUSION: Tachyplesin could effectively inhibit the proliferation of hepatocarcinoma cells, reverse the malignant morphological and ultrastructural characteristics, alter the levels of enzymes and antigens, regulate the expression of differentiation-associated oncogene and tumor suppressor gene, and induce hepatocarcinama cell differentiation.
引用
收藏
页码:1053 / 1058
页数:6
相关论文
共 48 条
[1]   Nuclear topography of the c-myc gene in human leukemic cells [J].
Bártová, E ;
Kozubek, S ;
Kozubek, M ;
Jirsová, P ;
Lukásová, E ;
Skalníková, M ;
Cafourková, A ;
Koutná, I .
GENE, 2000, 244 (1-2) :1-11
[2]  
Berkson J, 1999, FISHERIES, V24, P6, DOI 10.1577/1548-8446(1999)024&lt
[3]  
0006:THCTBF&gt
[4]  
2.0.CO
[5]  
2
[6]  
CAO JQ, 1998, SHIYONG AIZHENG ZAZH, V13, P105
[7]   Induction of differentiation in human hepatocarcinoma cells by isoverbascoside [J].
Chen, RC ;
Su, JH ;
Gao-Liang, OY ;
Cai, KX ;
Li, JQ ;
Xie, XG .
PLANTA MEDICA, 2002, 68 (04) :370-372
[8]  
CHEN RC, 2000, INT J MODERN CANC TH, V3, P29
[9]   Retinoic acid-induced cell cycle arrest of human myeloid cell lines is associated with sequential down-regulation of c-Myc and cyclin E and posttranscriptional up-regulation of p27Kip1 [J].
Dimberg, A ;
Bahram, F ;
Karlberg, I ;
Larsson, LG ;
Nilsson, K ;
Öberg, F .
BLOOD, 2002, 99 (06) :2199-2206
[10]  
Ebinuma H, 1999, INT J ONCOL, V15, P991