Porcine DAZL messenger RNA: Its expression and regulation during oocyte maturation

被引：14
作者：
Liu, Jinghe [1 ]
Linher, Katja [1 ]
Li, Julang [1 ]
机构：
[1] Univ Guelph, Dept Anim & Poultry Sci, Ontario Agr Coll, Guelph, ON N1G 2W1, Canada
来源：
MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2009年 / 311卷 / 1-2期
基金：
加拿大自然科学与工程研究理事会;
关键词：
DAZL; Meiosis; Oocyte; GDNF; EGF; IVM; MALE GERM-CELLS; DEVELOPMENTAL COMPETENCE; GROWTH-FACTOR; IN-VITRO; PREIMPLANTATION EMBRYOS; BINDING PROTEINS; STEM-CELLS; MOUSE; GENE; TRANSLATION;
D O I：
10.1016/j.mce.2009.06.003
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Deleted in Azoospermia-Like (DAZL) is known to play an important role during both spermatogenesis and oogenesis, as mutations in this gene may result in male and female sterility. In order to study the expression of DAZL in the pig, we cloned the full-length coding sequence and determined its mRNA and protein expression profile in the ovary and in oocytes undergoing in vitro maturation (IVM). Immunohistochemisty revealed that DAZL protein localizes to oocytes of both preantral and antral follicles. The expression in the oocytes was also confirmed by Western blot. Immunocytochemistry and real time RT-PCR showed that the DAZL transcript and protein accumulate during oocyte maturation. In addition, glial cell line-derived neurotrophic factor (GDNF), epidermal growth factor (EGF), and follicle-stimulating hormone (FSH) significantly stimulate DAZL expression in oocytes derived from antral follicles during IVM. Our results suggest that the porcine DAZL coding sequence is highly homologous to those reported for the human and mouse cDNAs, and that DAZL expression increases during oocyte maturation. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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页码：101 / 108
页数：8
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