Polymeric adsorbents with peptide pendants as artificial receptors for beta-lactam antibiotics by mimicking the binding sites of beta-lactamases

A series of polymeric adsorbents with peptide pendants were designed as the artificial receptors of beta-lactam antibiotics by mimicking the structures of binding site in beta-lactamases. Crosslinked poly(N, N-dimethyl acrylamide) gel as a carrier was prepared by suspension copolymerization of N, N-dimethyl acrylamide and N, N'-bisacryldiaminoethane and then functionalized with ethylenediamine after partial hydrolysis. Using solid-phase peptide synthesis with symmetrical anhydride of protected amino acid step by step, various peptide pendants were respectively anchored onto the functionalized carrier. The adsorption properties of these peptide-containing adsorbents for beta-lactam antibiotics such as ampicillin and cefotaxime were then studied. The results showed that only those adsorbents in which peptide chains contained more than one lysine residues could obviously adsorb both beta-lactams and that static interaction as well as hydrogen bond played an important role during the adsorption.