Amino Acid Substitutions and Intragenic Duplications of Bacillus sp PS3 Flagellin Cause Complementation of the Bacillus subtilis Flagellin Deletion Mutant

被引：2
作者：
Hayakawa, Jumpei [1 ]
Kambe, Takao [1 ]
Ishizuka, Morio [1 ]
机构：
[1] Chuo Univ, Dept Appl Chem, Fac Sci & Engn, Bunkyo Ku, Tokyo 1128551, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2009年 / 73卷 / 10期
关键词：
mutated flagellin; glycosylation; motility; thermophilic bacterium; Bacillus sp PS3; BACTERIAL FLAGELLA; ESCHERICHIA-COLI; GLYCOSYLATION; MOTILITY; FILAMENT; GENES; IDENTIFICATION; SEQUENCE; DOMAIN;
D O I：
10.1271/bbb.90428
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Bacillus sp. PS3 produces a glycosylated flagellin. In this study, a number of the glycosylated residues of the flagellin protein were found to be located in the central variable region of this protein. We also report that the motility defect of the Bacillus subtilis flagellin mutant was complemented by Bacillus sp. PS3 flagellin variants without glycosylation, which contained amino acid substitutions and intragenic duplications in the variable region of flagellin.
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收藏
页码：2348 / 2351
页数：4
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