Survivin enhances Aurora-B kinase activity and localizes Aurora-B in human cells

被引：110

作者：

Chen, J ^{[1
]}

Jin, S ^{[1
]}

Tahir, SK ^{[1
]}

Zhang, HC ^{[1
]}

Liu, XS ^{[1
]}

Sarthy, AV ^{[1
]}

McGonigal, TP ^{[1
]}

Liu, ZH ^{[1
]}

Rosenberg, SH ^{[1
]}

Ng, SC ^{[1
]}

机构：

[1] Abbott Labs, Canc Res, Abbott Pk, IL 60064 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2003年 / 278卷 / 01期

关键词：

D O I：

10.1074/jbc.M211119200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Survivin, one of the most tumor-specific gene products, has been implicated in both anti-apoptosis and cytokinesis. However, the mechanism by which survivin regulates these two different processes is still elusive. Here, we show that survivin binds to the catalytic domain of Aurora-B. We demonstrate that in the presence of survivin, Aurora-B phosphorylates histone H3 much more efficiently than in the absence of survivin in a cell-free system. Furthermore, we confirm that cells lacking survivin due to survivin antisense oligonucleotide-treatment have lower Aurora-B kinase activity, whereas cells overexpressing survivin have higher Aurora-B kinase activity. We also provide evidence that depletion of survivin by survivin antisense oligonucleotide treatment causes significant reduction of endogenous phosphorylated histone H3 and mislocalization of Aurora-B. These results indicate that survivin stimulates Aurora-B kinase activity and helps correctly target Aurora-B to its substrates during the cell cycle, thus providing a mechanism as to how survivin exerts its function in human cells.

引用

页码：486 / 490

页数：5

共 37 条

[1] INCENP binds the Aurora-related kinase AIRK2 and is required to target it to chromosomes, the central spindle and cleavage furrow [J].