Fatty acids by high-performance liquid chromatography and evaporative light-scattering detector

被引：70
作者：
Bravi, Elisabetta [1 ]
Perretti, Giuseppe [1 ]
Montanari, Luigi [1 ]
机构：
[1] Univ Perugia, Dept Econ & Food Sci, Sect Food Technol & Biotechnol, I-06126 Perugia, Italy
来源：
JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1134卷 / 1-2期
关键词：
HPLC; ELSD; fatty acids;
D O I：
10.1016/j.chroma.2006.09.007
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A high-performance liquid chromatographic (HPLC) separation method with an evaporative light-scattering detector (ELSD) has been developed for the separation and quantitative analysis of fatty acid methyl esters (FAME) in three different oils. Reverse-phased C(18) HPLC separation of 13 FAME is achieved using a methanol/water eluent mixture. The retention times (RT) reflect the elution behavior of these compounds on C(18) reversed-phase HPLC. The proposed method is tested on: soybean oil (Glycine max L.) as reference sample, rice bran oil (Oryza sativa L.), pumpkin seed oil (Cucurbita pepo L.) and algal oil (Arthrospira platensis Nordst.). (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：210 / 214
页数：5
相关论文
共 50 条
[31]   EVAPORATIVE LIGHT-SCATTERING DETECTOR FOR ANALYSIS OF NATURAL PRODUCTS [J].
Morais Urano, Raquel P. ;
Rodrigues, Fabiana T. ;
Berlinck, Roberto G. S. .
QUIMICA NOVA, 2012, 35 (06) :1198-1208
[32]   Analysis of neomycin sulfate and framycetin sulfate by high-performance liquid chromatography using evaporative light scattering detection [J].
Clarot, I ;
Regazzeti, A ;
Auzeil, N ;
Laadani, F ;
Citton, M ;
Netter, P ;
Nicolas, A .
JOURNAL OF CHROMATOGRAPHY A, 2005, 1087 (1-2) :236-244
[33]   Separation and identification of neutral cereal lipids by normal phase high-performance liquid chromatography, using evaporative light-scattering and electrospray mass spectrometry for detection [J].
Rocha, Joao M. ;
Kalo, Paavo J. ;
Ollilainen, Velimatti ;
Malcata, F. Xavier .
JOURNAL OF CHROMATOGRAPHY A, 2010, 1217 (18) :3013-3025
[34]   Calibration of an Evaporative Light-Scattering Detector for the Universal Quantitative Analyses in Liquid Chromatography—Application to the Determination of Triacylglycerols in Cucurbitaceous Oils [J].
Sylvie Heron ;
Marie Geneviève Maloumbi ;
Thomas Silou ;
Eric Verette ;
Michel Dreux ;
Alain Tchapla .
Food Analytical Methods, 2010, 3 :67-74
[35]   Development of a simple high performance liquid chromatography (HPLC)/evaporative light scattering detector (ELSD) method to determine Polysorbate 80 in a pharmaceutical formulation [J].
Mondal, Bikash ;
Kote, Mahesh ;
Lunagariya, Chandrakant ;
Patel, Milan .
SAUDI PHARMACEUTICAL JOURNAL, 2020, 28 (03) :325-328
[36]   Application of High Performance Liquid Chromatography/Evaporative Light Scattering Detector (HPLC/ELSD) for the study and the fractionation of milk fat triacylglycerols [J].
Povolo, M. ;
Contarini, G. .
RIVISTA ITALIANA DELLE SOSTANZE GRASSE, 2012, 89 (03) :184-192
[37]   Simultaneous determination of major bioactive components in Qingkailing injection by high-performance liquid chromatography with evaporative light scattering detection [J].
Yan, SK ;
Xin, WF ;
Luo, GA ;
Wang, YM ;
Cheng, YY .
CHEMICAL & PHARMACEUTICAL BULLETIN, 2005, 53 (11) :1392-1395
[38]   Fingerprint quality detection of Solanum nigrum using high-performance liquid chromatography--evaporative light scattering detection [J].
Wang, Liye ;
Gao, Guilong ;
Bai, Yang ;
Luo, Wenmin ;
Lin, Changhu ;
Jia, Qiang .
PHARMACEUTICAL BIOLOGY, 2011, 49 (06) :595-601
[39]   EVAPORATIVE LIGHT-SCATTERING MASS DETECTION FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF SUCROSE POLYESTER BLENDS IN COOKING OILS [J].
CHASE, GW ;
AKOH, CC ;
EITENMILLER, RR .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1994, 71 (11) :1273-1276
[40]   Calibration of an Evaporative Light-Scattering Detector for the Universal Quantitative Analyses in Liquid Chromatography-Application to the Determination of Triacylglycerols in Cucurbitaceous Oils [J].
Heron, Sylvie ;
Maloumbi, Marie Genevieve ;
Silou, Thomas ;
Verette, Eric ;
Dreux, Michel ;
Tchapla, Alain .
FOOD ANALYTICAL METHODS, 2010, 3 (02) :67-74
12345