Fatty acids by high-performance liquid chromatography and evaporative light-scattering detector

被引：68
|
作者：
Bravi, Elisabetta [1 ]
Perretti, Giuseppe [1 ]
Montanari, Luigi [1 ]
机构：
[1] Univ Perugia, Dept Econ & Food Sci, Sect Food Technol & Biotechnol, I-06126 Perugia, Italy
来源：
JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1134卷 / 1-2期
关键词：
HPLC; ELSD; fatty acids;
D O I：
10.1016/j.chroma.2006.09.007
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A high-performance liquid chromatographic (HPLC) separation method with an evaporative light-scattering detector (ELSD) has been developed for the separation and quantitative analysis of fatty acid methyl esters (FAME) in three different oils. Reverse-phased C(18) HPLC separation of 13 FAME is achieved using a methanol/water eluent mixture. The retention times (RT) reflect the elution behavior of these compounds on C(18) reversed-phase HPLC. The proposed method is tested on: soybean oil (Glycine max L.) as reference sample, rice bran oil (Oryza sativa L.), pumpkin seed oil (Cucurbita pepo L.) and algal oil (Arthrospira platensis Nordst.). (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：210 / 214
页数：5
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