A genetic analysis of crystal growth

被引：317
作者：
Brown, S
Sarikaya, M
Johnson, E
机构：
[1] Univ Copenhagen, Dept Mol Cell Biol, DK-1353 Copenhagen K, Denmark
[2] Univ Washington, Dept Mat Sci & Engn, Seattle, WA 98195 USA
[3] Univ Copenhagen, Niels Bohr Inst, Orsted Lab, DK-2100 Copenhagen O, Denmark
来源：
JOURNAL OF MOLECULAR BIOLOGY | 2000年 / 299卷 / 03期
关键词：
gold; crystal morphology; peptide display;
D O I：
10.1006/jmbi.2000.3682
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The regulation of crystal morphology by proteins is often observed in biology. It is a central feature in the formation of hard tissues such as bones, teeth and mollusc shells. We have developed a genetic system in the bacterium Escherichia coli to study the protein-mediated control of crystal growth. We have used the crystallization of gold as a model system and found polypeptides that control the morphology of the resulting gold crystals. Analysis of the crystallization process influenced by these polypeptides indicates they act catalytically by an acid mechanism. Our results suggest that the concepts and methods of microbial genetics are general and can be applied to substances not commonly found in biological systems. (C) 2000 Academic Press.
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页码：725 / 735
页数：11
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