Production and characterization of an intracellular bioflocculant by Chryseobacterium daeguense W6 cultured in low nutrition medium

被引：143

作者：

Liu, WeiJie ^{[1
]}

Wang, Kai ^{[1
]}

Li, BaoZhen ^{[1
]}

Yuan, HongLi ^{[1
]}

Yang, JinShui ^{[1
]}

机构：

[1] China Agr Univ, State Key Lab Agrobiotechnol, Key Lab Agromicrobial Resource & Applicat, Ctr Biomass Engn,Minist Agr,Coll Biol Sci, Beijing 100193, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 03期

基金：

美国国家科学基金会;

关键词：

Chryseobacterium daeguense; Flocculating rate; Flocculant; Intracellular bioflocculant; Extracellular polymeric substances; WATER; FLOCCULANTS;

D O I：

10.1016/j.biortech.2009.08.108

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

A novel intracellular bioflocculant (named MBF-W6) produced by Chryseobacterium daeguense W6 cultured in low nutrition medium was investigated in this study. The effects of carbon source, nitrogen source, C/N ratio, initial pH, inoculum size, culture temperature and shaking speed on MBF-W6 production were studied. Chemical analysis. showed that the purified MBF-W6 was mainly composed of 32.4% protein, 13.1% polysaccharide and 6.8% nucleic acid. Fourier-transform infrared (FTIR) spectroscopy indicated the presence of carboxyl, hydroxyl, and methoxyl groups. The elemental analysis of purified MBF-W6 revealed that the mass proportion of C, H, O, N and S was 40.92:6.53:44.01:8.53:1.01 (w/w) correspondingly. MBF-W6 had good flocculating rate in Kaolin suspension without any cation addition. The highest flocculating rate of 96.9% was achieved under the optimal conditions (bioflocculant dosage 1.2 mg l(-1), pH 5.6 and temperature 15 degrees C). (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1044 / 1048

页数：5

共 23 条

[1] Extraction of extracellular polymeric substances from aerobic granule with compact interior structure [J].

Adav, Sunil S. ;

Lee, Duu-Jong .

JOURNAL OF HAZARDOUS MATERIALS, 2008, 154 (1-3) :1120-1126

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3]

Chaplin M.F., 1994, CARBOHYDRATE ANAL

[4] THE ROLE OF EXTRACELLULAR POLYSACCHARIDES IN BIOFILMS [J].

CHRISTENSEN, BE .

JOURNAL OF BIOTECHNOLOGY, 1989, 10 (3-4) :181-201

[5] Production of a bioflocculant by Aspergillus parasiticus and its application in dye removal [J].

Deng, SB ;

Yu, G ;

Ting, YP .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2005, 44 (04) :179-186

[6] Purification and characterization of an extracellular polysaccharide from haloalkalophilic Bacillus sp I-450 [J].

Ganesh Kumar, C ;

Joo, HS ;

Choi, JW ;

Koo, YM ;

Chang, CS .

ENZYME AND MICROBIAL TECHNOLOGY, 2004, 34 (07) :673-681

[7] Bioflocculant production by culture of Serratia ficaria and its application in wastewater treatment [J].

Gong, Wen-Xin ;

Wang, Shu-Guang ;

Sun, Xue-Fei ;

Liu, Xian-Wei ;

Yue, Qin-Yan ;

Gao, Bao-Yu .

BIORESOURCE TECHNOLOGY, 2008, 99 (11) :4668-4674

[8]

GUSSOW D, 1989, NUCLEIC ACIDS RES, V17, P4000

[9] Identification of a novel bioflocculant from a newly isolated Corynebacterium glutamicum [J].

He, N ;

Li, Y ;

Chen, J ;

Lun, SY .

BIOCHEMICAL ENGINEERING JOURNAL, 2002, 11 (2-3) :137-148

[10] MICROBIAL FLOCCULANTS .1. SCREENING FOR AND CHARACTERISTICS OF MICROBIAL FLOCCULANTS [J].

KURANE, R ;

TAKEDA, K ;

SUZUKI, T .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1986, 50 (09) :2301-2307

← 1 2 3 →