Our preliminary findings indicated that at 4 degrees C, a High Voltage Electrostatic Field (HVEF) of 100 kV/m can effectively extend the shelf life of fish for up to 2 days. This study further investigated changes in the freshness of tilapia meat after using various HVEF treatments: 300, 600, or 900 kV/m. Both the VBN and K-values of the untreated tilapia meat increased during the storage period, eventually exceeding the hygienic standard on the 6th day, with values of 60% and 25 mg/100 g, respectively. However, in fish treated with 600 kV/m HVEF, the K value approached 60% around the 8th day; fish treated with 300 kV/m HVEF had a VBN of only 20.47 mg/100 g. This demonstrated the effectiveness of HVEF in preserving freshness. In untreated fish, the total plate counts (TPC) reached 4.02 x 10(5) CFU/g meat by the 8th day, while fish treated with an HVEF of 900 kV/m still had not exceeded the sanitary standard in that same time period (3.45 x 10(5) CFU/g meat). In summary, an HVEF with 600 kV/m electric field strength or more can enhance food preservation, as it effectively delays ATP degradation, protein denaturation, lipid oxidation, and microbiological growth. (C) 2015 Elsevier Ltd. All rights reserved.