Vitamin C Enhances the Generation of Mouse and Human Induced Pluripotent Stem Cells

被引：894

作者：

Esteban, Miguel Angel ^{[1
,2
]}

Wang, Tao ^{[1
,2
]}

Qin, Baoming ^{[1
,2
]}

Yang, Jiayin ^{[1
,2
]}

Qin, Dajiang ^{[1
,2
]}

Cai, Jinglei ^{[1
,2
]}

Li, Wen ^{[1
,2
]}

Weng, Zhihui ^{[1
,2
]}

Chen, Jiekai ^{[1
,2
]}

Ni, Su ^{[1
,2
]}

Chen, Keshi ^{[1
,2
]}

Li, Yuan ^{[1
,2
]}

Liu, Xiaopeng ^{[1
,2
]}

Xu, Jianyong ^{[1
,2
]}

Zhang, Shiqiang ^{[1
,2
]}

Li, Feng ^{[1
,2
]}

He, Wenzhi ^{[1
,2
]}

Labuda, Krystyna ^{[3
]}

Song, Yancheng ^{[4
]}

Peterbauer, Anja ^{[5
]}

Wolbank, Susanne ^{[3
]}

Redl, Heinz ^{[3
]}

Zhong, Mei ^{[6
]}

Cai, Daozhang ^{[4
]}

Zeng, Lingwen ^{[1
,2
]}

Pei, Duanqing ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Guangzhou Inst Biomed, S China Inst Stem Cell Biol & Regenerat Med, Stem Cell & Canc Biol Grp,Key Lab Regenerat Biol, Guangzhou 510663, Guangdong, Peoples R China

[2] Chinese Acad Sci, Guangzhou Inst Hlth, Guangzhou 510663, Guangdong, Peoples R China

[3] Ludwig Boltzmann Inst Clin & Expt Traumatol, A-1200 Vienna, Austria

[4] Sun Yat Sen Univ, Affiliated Hosp 3, Guangzhou 510630, Guangdong, Peoples R China

[5] Red Cross Blood Transfus Serv Upper Austria, A-4020 Linz, Austria

[6] Nanfang Hosp, Ctr Prenatal & Hereditary Dis Diag, Guangzhou 510515, Guangdong, Peoples R China

来源：

CELL STEM CELL | 2010年 / 6卷 / 01期

基金：

中国国家自然科学基金;

关键词：

DEMETHYLASES; FIBROBLASTS; EFFICIENCY; INDUCTION; LIMITS; MYC;

D O I：

10.1016/j.stem.2009.12.001

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. However, the low efficiency and slow kinetics of the reprogramming process have hampered progress with this technology. Here we report that a natural compound, vitamin C (Vc), enhances iPSC: generation from both mouse and human somatic cells. Vc acts at least in part by alleviating cell senescence, a recently identified roadblock for reprogramming. In addition, Vc accelerates gene expression changes and promotes the transition of pre-iPSC colonies to a fully reprogrammed state. Our results therefore highlight a straightforward method for improving the speed and efficiency of iPSC generation and provide additional insights into the mechanistic basis of the reprogramming process.

引用

页码：71 / 79

页数：9

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