Development of an enhanced separation of erythromycin and its related substances by liquid chromatography

被引：14

作者：

Deubel, A. ^{[1
]}

Holzgrabe, U. ^{[1
]}

机构：

[1] Univ Wurzburg, Inst Pharm & Food Chem, D-97074 Wurzburg, Germany

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2007年 / 43卷 / 02期

关键词：

HPLC; erythromycin; European pharmacopoeia;

D O I：

10.1016/j.jpba.2006.07.044

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new HPLC-UV method for the determination of the impurity profile of erythromycin is developed. In contrast to the liquid chromatography described in the European Pharmacopoeia the analysis could be performed at a temperature of 25 degrees C. Erythromycin samples were analysed on an endcapped RP phase with cyanopropyl groups on the surface using gradient elution with 32 mM potassium phosphate buffer pH 8.0 and acetonitrile/methanol (75:25). The aforementioned method shows clear improvements compared to the actual method of the European Pharmacopoeia, which is less selective and sensitive. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：493 / 498

页数：6

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