Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster

被引:222
作者
Colinet, Herve [1 ,2 ]
Lee, Siu Fai [1 ]
Hoffmann, Ary [1 ]
机构
[1] Univ Melbourne, Inst Bio21, Dept Genet, Ctr Environm Stress & Adaptat Res, Parkville, Vic 3010, Australia
[2] Catholic Univ Louvain, Biodivers Res Ctr, Unite Ecol & Biogeog, B-3000 Louvain, Belgium
基金
澳大利亚研究理事会;
关键词
cold stress; Drosophila melanogaster; gene expression; Hsp; recovery; SARCOPHAGA-CRASSIPALPIS; FLESH FLY; UP-REGULATION; KNOCKDOWN RESISTANCE; CELLULAR-RESPONSES; OXIDATIVE STRESS; LOW-TEMPERATURES; THERMAL-STRESS; PUPAL DIAPAUSE; PROTEINS;
D O I
10.1111/j.1742-4658.2009.07470.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A common physiological response of organisms to environmental stresses is the increase in expression of heat shock proteins (Hsps). In insects, this process has been widely examined for heat stress, but the response to cold stress has been far less studied. In the present study, we focused on 11 Drosophila melanogaster Hsp genes during the stress exposure and recovery phases. The temporal gene expression of adults was analyzed during 9 h of cold stress at 0 degrees C and during 8 h of recovery at 25 degrees C. Increased expression of some, but not all, Hsp genes was elicited in response to cold stress. The transcriptional activity of Hsp genes was not modulated during the cold stress, and peaks of expression occurred during the recovery phase. On the basis of their response, we consider that Hsp60, Hsp67Ba and Hsc70-1 are not cold-inducible, whereas Hsp22, Hsp23, Hsp26, Hsp27, Hsp40, Hsp68, Hsp70Aa and Hsp83 are induced by cold. This study suggests the importance of the recovery phase for repairing chilling injuries, and highlights the need to further investigate the contributions of specific Hsp genes to thermal stress responses. Parallels are drawn between the stress response networks resulting from heat and cold stress.
引用
收藏
页码:174 / 185
页数:12
相关论文
共 69 条
[21]   Opposing clines for high and low temperature resistance in Drosophila melanogaster [J].
Hoffmann, AA ;
Anderson, A ;
Hallas, R .
ECOLOGY LETTERS, 2002, 5 (05) :614-618
[22]  
Hoffmann AA, 2002, EVOLUTION, V56, P1068, DOI 10.1111/j.0014-3820.2002.tb01418.x
[23]   Flight muscle resting potential and species-specific differences in chill-coma [J].
Hosler, JS ;
Burns, JE ;
Esch, HE .
JOURNAL OF INSECT PHYSIOLOGY, 2000, 46 (05) :621-627
[24]   Expression profiling and validation of reference gene candidates in immune relevant tissues and cells from Atlantic salmon (Salmo salar L.) [J].
Ingerslev, HC ;
Pettersen, EF ;
Jakobsen, RA ;
Petersen, CB ;
Wergeland, HI .
MOLECULAR IMMUNOLOGY, 2006, 43 (08) :1194-1201
[25]   COLD SHOCK ELICITS EXPRESSION OF HEAT-SHOCK PROTEINS IN THE FLESH FLY, SARCOPHAGA-CRASSIPALPIS [J].
JOPLIN, KH ;
YOCUM, GD ;
DENLINGER, DL .
JOURNAL OF INSECT PHYSIOLOGY, 1990, 36 (11) :825-834
[26]   Decrease in expression of beta-tubulin and microtubule abundance in flight muscles during diapause in adults of Culex pipiens [J].
Kim, M. ;
Denlinger, D. L. .
INSECT MOLECULAR BIOLOGY, 2009, 18 (03) :295-302
[27]   The 70 kDa Heat Shock Protein Assists during the Repair of Chilling Injury in the Insect, Pyrrhocoris apterus [J].
Kostal, Vladimir ;
Tollarova-Borovanska, Michaela .
PLOS ONE, 2009, 4 (02)
[28]   Cold exposure and associated metabolic changes in adult tropical beetles exposed to fluctuating thermal regimes [J].
Lalouette, L. ;
Kostal, V. ;
Colinet, H. ;
Gagneul, D. ;
Renault, D. .
FEBS JOURNAL, 2007, 274 (07) :1759-1767
[29]   Mechanism of regulation of Hsp70 chaperones by DnaJ cochaperones [J].
Laufen, T ;
Mayer, MP ;
Beisel, C ;
Klostermeier, D ;
Mogk, A ;
Reinstein, J ;
Bukau, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (10) :5452-5457
[30]   Quantitative transcript imaging in normal and heat-shocked Drosophila embryos by using high-density oligonucleotide arrays [J].
Leemans, R ;
Egger, B ;
Loop, T ;
Kammermeier, L ;
He, HQ ;
Hartmann, B ;
Certa, U ;
Hirth, F ;
Reichert, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (22) :12138-12143