A new simple and sensitive fluorometric method for the determination of hydroxyl radical and its application

A simple and sensitive fluorometric method to detect the hydroxyl radical is described. This method employs the reaction between .OH and dimethyl sulfoxide (DMSO) to generate quantitatively formaldehyde, which then reacts with ammonia and 1,3-cyclohexanedione (CHD) at pH 4.5. The product produces the characteristic fluorescence with its excitation and emission wavelength at 400.4 and 452.3 nm, respectively. The quantitative analysis of hydroxyl radical can be done through the determination of the fluorescence intensity. By the developed method, the scavenging abilities of some amino acids and flavonoids on hydroxyl radical are studied. (C) 2002 Published by Elsevier Science B.V.