Development of a one-run real-time PCR detection system for pathogens associated with bovine respiratory disease complex

被引:79
作者
Kishimoto, Mai [1 ]
Tsuchiaka, Shinobu [1 ,2 ]
Rahpaya, Sayed Samim [1 ,2 ]
Hasebe, Ayako [3 ]
Otsu, Keiko [3 ]
Sugimura, Satoshi [4 ]
Kobayashi, Suguru [4 ]
Komatsu, Natsumi [4 ]
Nagai, Makoto [1 ,6 ]
Omatsu, Tsutomu [1 ,2 ]
Naoi, Yuki [1 ]
Sano, Kaori [1 ]
Okazaki-Terashima, Sachiko [1 ]
Oba, Mami [1 ]
Katayama, Yukie [1 ]
Sato, Reiichiro [5 ]
Asai, Tetsuo [2 ]
Mizutani, Tetsuya [1 ,2 ]
机构
[1] Tokyo Univ Agr & Technol, Res & Educ Ctr Prevent Global Infect Dis Anim, 3-5-8 Saiwai, Fuchu, Tokyo 1838509, Japan
[2] Gifu Univ, United Grad Sch Vet Sci, 1-1 Yanagito, Gifu 5011193, Japan
[3] Gifu Cent Livestock Hyg Serv Ctr, 4-2-22 Imarnine, Gifu 5008388, Japan
[4] Tokyo Univ Agr & Technol, Inst Agr, 3-5-8 Saiwai, Fuchu, Tokyo 1838509, Japan
[5] Azabu Univ, Sch Vet Med, Chuo Ku, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 2525201, Japan
[6] Ishikawa Prefectural Univ, Fac Bioresources & Environm Sci, 1-308 Suematsu, Nonoichi, Ishikawa 9218836, Japan
关键词
bovine respiratory disease complex; diagnosis; taqMan real-time PCR; INFLUENZA D VIRUS; SYNCYTIAL VIRUS; RT-PCR; BACTERIAL PATHOGENS; BEEF-CATTLE; INFECTION; ASSAY; HERPESVIRUS-1; EPIDEMIOLOGY; MORTALITY;
D O I
10.1292/jvms.16-0489
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Bovine respiratory disease complex (BRDC) is frequently found in cattle worldwide. The etiology of BRDC is complicated by infections with multiple pathogens, making identification of the causal pathogen difficult. Here, we developed a detection system by applying TaqMan real-time PCR (Dembo respiratory-PCR) to screen a broad range of microbes associated with BRDC in a single run. We selected 16 bovine respiratory pathogens (bovine viral diarrhea virus, bovine coronavirus, bovine parainfluenza virus 3, bovine respiratory syncytial virus, influenza D virus, bovine rhinitis A virus, bovine rhinitis B virus, bovine herpesvirus 1, bovine adenovirus 3, bovine adenovirus 7, Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, Trueperella I pyogenes, Mycoplasma bovis and Ureaplasma diversum) as detection targets and designed novel specific primer-probe sets for nine of them. The assay performance was assessed using standard curves from synthesized DNA. In addition, the sensitivity of the assay was evaluated by spiking solutions extracted from nasal swabs that were negative by Dembo respiratory-PCR for nucleic acids of pathogens or synthesized DNA. All primer-probe sets showed high sensitivity. In this study, a total of 40 nasal swab samples from cattle on six farms were tested by Dembo respiratory-PCR. Dembo respiratory-PCR can be applied as a screening system with wide detection targets.
引用
收藏
页码:517 / 523
页数:7
相关论文
共 33 条
  • [1] ALLEN JW, 1992, CAN J VET RES, V56, P177
  • [2] Real Time RT-PCR for the detection and quantitation of bovine respiratory syncytial virus
    Boxus, M
    Letellier, C
    Kerkhofs, P
    [J]. JOURNAL OF VIROLOGICAL METHODS, 2005, 125 (02) : 125 - 130
  • [3] The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
    Bustin, Stephen A.
    Benes, Vladimir
    Garson, Jeremy A.
    Hellemans, Jan
    Huggett, Jim
    Kubista, Mikael
    Mueller, Reinhold
    Nolan, Tania
    Pfaffl, Michael W.
    Shipley, Gregory L.
    Vandesompele, Jo
    Wittwer, Carl T.
    [J]. CLINICAL CHEMISTRY, 2009, 55 (04) : 611 - 622
  • [4] Detection of Influenza D Virus among Swine and Cattle, Italy
    Chiapponi, Chiara
    Faccini, Silvia
    De Mattia, Aurora
    Baioni, Laura
    Barbieri, Ilaria
    Rosignoli, Carlo
    Nigrelli, Arrigo
    Foni, Emanuela
    [J]. EMERGING INFECTIOUS DISEASES, 2016, 22 (02) : 352 - 354
  • [5] Cocirculation of Two Distinct Genetic and Antigenic Lineages of Proposed Influenza D Virus in Cattle
    Collin, Emily A.
    Sheng, Zizhang
    Lang, Yuekun
    Ma, Wenjun
    Hause, Ben M.
    Li, Feng
    [J]. JOURNAL OF VIROLOGY, 2015, 89 (02) : 1036 - 1042
  • [6] Influenza D virus infection in Mississippi beef cattle
    Ferguson, Lucas
    Eckard, Laura
    Epperson, William B.
    Long, Li-Ping
    Smith, David
    Huston, Carla
    Genova, Suzanne
    Webby, Richard
    Wan, Xiu-Feng
    [J]. VIROLOGY, 2015, 486 : 28 - 34
  • [7] Fulton RW, 2012, CAN VET J, V53, P754
  • [8] Diseases and pathogens associated with mortality in Ontario beef feedlots
    Gagea, MI
    Bateman, KG
    van Dreumel, T
    McEwen, BJ
    Carman, S
    Archambault, M
    Shanahan, RA
    Caswell, JL
    [J]. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2006, 18 (01) : 18 - 28
  • [9] Effect of infection with bovine respiratory syncytial virus on pulmonary clearance of an inhaled antigen in calves
    Gershwin, Laurel J.
    Gunther, Robert A.
    Hornof, William J.
    Larson, Richard F.
    [J]. AMERICAN JOURNAL OF VETERINARY RESEARCH, 2008, 69 (03) : 416 - 422
  • [10] Single Pathogen Challenge with Agents of the Bovine Respiratory Disease Complex
    Gershwin, Laurel J.
    Van Eenennaam, Alison L.
    Anderson, Mark L.
    McEligot, Heather A.
    Shao, Matt X.
    Toaff-Rosenstein, Rachel
    Taylor, Jeremy F.
    Neibergs, Holly L.
    Womack, James
    [J]. PLOS ONE, 2015, 10 (11):