Silymarin and dimercaptosuccinic acid ameliorate lead-induced nephrotoxicity and genotoxicity in rats

被引：27
作者：
Alcaraz-Contreras, Y. [1 ]
Mendoza-Lozano, R. P. [1 ]
Martinez-Alcaraz, E. R. [1 ]
Martinez-Alfaro, M. [1 ]
Gallegos-Corona, M. A. [2 ]
Ramirez-Morales, M. A. [1 ]
Vazquez-Guevara, M. A. [3 ]
机构：
[1] Univ Guanajuato, Dept Farm, Div Ciencias Nat & Exactas, Noria Alta S-N, Guanajuato 36050, Mexico
[2] Univ Autonoma Queretaro, Fac Med, Queretaro, Mexico
[3] Univ Guanajuato, Dept Quim, Div Ciencias Nat & Exactas, Guanajuato, Mexico
来源：
HUMAN & EXPERIMENTAL TOXICOLOGY | 2016年 / 35卷 / 04期
关键词：
Lead acetate; dimercaptosuccinic acid; silymarin; kidney toxicity; comet assay; INDUCED OXIDATIVE STRESS; THERAPEUTIC POTENTIALS; SILYBUM-MARIANUM; CALCIUM DISODIUM; N-ACETYLCYSTEINE; MILK THISTLE; SUCCIMER; LIVER; ANTIOXIDANT; TOXICITY;
D O I：
10.1177/0960327115591373
中图分类号：
R99 [毒物学（毒理学）];
学科分类号：
100405 ;
摘要：
We studied the effect of silymarin and dimercaptosuccinic acid (DMSA), a chelating agent that was administered individually or in combination against lead (Pb) toxicity in rats. Wistar rats (200 +/- 20) were randomly divided into five groups. Group A served as a control. Groups B-E were exposed to 2000 ppm of lead acetate in drinking water for 8 weeks. Group B served as a positive control. Group C received silymarin (100 mg kg(-1) orally) for 8 weeks. Group D received DMSA (75 mg kg(-1) orally) once daily for the last 5 days of treatment. Group E received DMSA and silymarin as groups C and D, respectively. The effect of Pb was evaluated and accordingly the treatments on blood lead levels (BLLs), renal system, and genotoxic effects were calculated using comet assay. The BLLs were significantly increased following the exposition of lead acetate. The administration of silymarin and DMSA provided reduction in BLLs. Silymarin and DMSA provided significant protection on the genotoxic effect of Pb. The toxic effect of Pb on kidneys was also studied. Our data suggest that silymarin and DMSA improve the renal histopathological lesions.
引用
收藏
页码：398 / 403
页数：6
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