Purification of Candida rugosa lipase using agarose mercurial column

被引：2

作者：

Tai, DF ^{[1
]}

Chang, YF

Chiou, TW

机构：

[1] Natl Dong Hwa Univ, Dept Chem, Hualien 974, Taiwan

[2] Natl Dong Hwa Univ, Inst Biotechnol, Hualien 974, Taiwan

来源：

JOURNAL OF THE CHINESE CHEMICAL SOCIETY | 2002年 / 49卷 / 05期

关键词：

lipase; agarose mercurial column; kinetic resolution; 2-phenylpropionic acid;

D O I：

10.1002/jccs.200200135

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Candida rugosa lipase from Sigma was subjected to an agarose mercurial column, and the three fractions thus collected were further desalted using a Sephadex G-25 column. The purification of these isozymes increases their catalytic activities toward the hydrolysis of triacetin. An improved resolution on the hydrolysis of 2-chloroethyl 2-phenylpropionate was also observed by utilizing one of the isolated isozymes CRL II.

引用

页码：957 / 960

页数：4

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