Optimization of stripline-based microfluidic chips for high-resolution NMR

被引：63

作者：

Bart, J. ^{[1
]}

Janssen, J. W. G. ^{[2
]}

van Bentum, P. J. M. ^{[2
]}

Kentgens, A. P. M. ^{[2
]}

Gardeniers, J. G. E. ^{[1
]}

机构：

[1] Univ Twente, MESA Inst Nanotechnol, NL-7500 AE Enschede, Netherlands

[2] Radboud Univ Nijmegen, Inst Mol & Mat, NL-6525 ED Nijmegen, Netherlands

来源：

JOURNAL OF MAGNETIC RESONANCE | 2009年 / 201卷 / 02期

关键词：

Stripline; Microcoil; Microfluidic; Lab-on-a-chip; NUCLEAR-MAGNETIC-RESONANCE; HIGH-RESISTIVITY SILICON; H-1-NMR SPECTROSCOPY; ANALYSIS SYSTEMS; PROBE; MICROCOILS; SUBSTRATE; DESIGN;

D O I：

10.1016/j.jmr.2009.09.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We here report on the optimization, fabrication and experimental characterization of a stripline-based microfluidic NMR probe, realized in a silicon substrate. The stripline geometry was modelled in respect of rf-homogeneity, sensitivity and spectral resolution. Using these models, optimal dimensional ratios were found, which hold for every sample size. Based on the optimized parameters, a simple integrated stripline-based microfluidic chip was realized. The fabrication of this chip is described in detail. We achieved a sensitivity of 0.47 nmol/root Hz and a resolution of 0.7 Hz. The rf-homlogeneity (A(810 degrees)/A(90 degrees).) was 76% and was proved to be suitable for 2D-NMR analysis of glucose. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：175 / 185

页数：11

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