The influence of salt type on the retention of bovine serum albumin in ion-exchange chromatography

被引:7
作者
Al-Jibbouri, Sattar [1 ]
机构
[1] Tech Univ Denmark, SEP, IVC, Eng Res Ctr,Dept Chem Engn, DK-2800 Lyngby, Denmark
关键词
salt types; retention volume; steric mass action; ion-exchange; BSA;
D O I
10.1016/j.chroma.2006.10.079
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In this paper, an analysis of the influence of the salt types, NaCl, NaCH3COO, Na2SO4 and Na3C6H5O7, on the isocratic retention behaviour of bovine serum albumin (BSA) on two anion-exchangers media (Source 30Q and TSK Gel Super Q 5 PW) has been presented. The retention data demonstrated that the mechanism of protein retention in ion-exchange chromatography (IEC) involves interactions between the protein solute, the mobile phase constituents and the stationary phase. The effect of protein activity coefficient in the mobile phase on the protein retention volumes is verified. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:57 / 62
页数:6
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