Oxalate and Oxalate Oxidase in Malt

被引:19
作者
Kanauchi, Makoto [2 ]
Milet, Jerome [1 ]
Bamforth, Charles W. [1 ]
机构
[1] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA
[2] Miyagi Univ, Dept Food Management, Taihaku Ku, Sendai, Miyagi 9820215, Japan
关键词
barley; malting; mashing; oxalate oxidase; oxalic acid; CALCIUM-OXALATE; PROTEIN; GERMIN; PURIFICATION; STORAGE; BARLEY; PLANT;
D O I
10.1002/j.2050-0416.2009.tb00374.x
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Barley kernels contain a single oxalate oxidase located in the embryo and aleurone. It is already present in substantial quantities in unmalted grain and increases in activity during germination. It displays a very broad pH optimum: the optimum was at pH 4.0, but the enzyme still displayed more than 50% of its activity at pH 7. Oxalate oxidase is highly resistant to heat. However, its low affinity for oxygen suggests that it probably does not play a major role in the consumption of oxygen in mashing. The decrease in oxalic acid levels late in germination may be a result of oxalate oxidase action. Oxalic acid was not detected in raw barley.
引用
收藏
页码:232 / 237
页数:6
相关论文
共 18 条
[1]   Storage of Malt, Thiol Oxidase, and Brewhouse Performance [J].
Bamforth, C. W. ;
Roza, J. R. ;
Kanauchi, M. .
JOURNAL OF THE AMERICAN SOCIETY OF BREWING CHEMISTS, 2009, 67 (02) :89-94
[2]   A two-substrate kinetic analysis of lipoxygenase in malt [J].
Biawa, JP ;
Bamforth, CW .
JOURNAL OF CEREAL SCIENCE, 2002, 35 (01) :95-98
[3]  
Burger M., 1949, P AM SOC BREW CHEM, V7, DOI [10.1080/00960845.1949.12006272, DOI 10.1080/00960845.1949.12006272]
[4]  
BURGER M, 1956, P AM SOC BREW CHEM, V14, P169
[5]   Specific and constitutive expression of oxalate oxidase during the ageing of leaf sheaths of ryegrass stubble [J].
Davoine, C ;
Le Deunff, E ;
Ledger, N ;
Avice, JC ;
Billard, JP ;
Dumas, B ;
Huault, C .
PLANT CELL AND ENVIRONMENT, 2001, 24 (10) :1033-1043
[6]   Microbial relatives of the seed storage proteins of higher plants: Conservation of structure and diversification of function during evolution of the cupin superfamily [J].
Dunwell, JM ;
Khuri, S ;
Gane, PJ .
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2000, 64 (01) :153-+
[7]   CALCIUM-OXALATE FORMATION IS A RAPID AND REVERSIBLE PROCESS IN LEMNA-MINOR-L [J].
FRANCESCHI, VR .
PROTOPLASMA, 1989, 148 (2-3) :130-137
[8]   SIGNAL RESISTANCE OF A SOLUBLE-PROTEIN TO ENZYMIC PROTEOLYSIS - AN UNORTHODOX APPROACH TO THE ISOLATION AND PURIFICATION OF GERMIN, A RARE GROWTH-RELATED PROTEIN [J].
GRZELCZAK, ZF ;
LANE, BG .
CANADIAN JOURNAL OF BIOCHEMISTRY AND CELL BIOLOGY, 1984, 62 (12) :1351-1353
[9]  
Hodgkinson A., 1977, OXALIC ACID BIOL MED
[10]   CELLULASES OF PLANT AND MICROBIAL ORIGIN IN GERMINATING BARLEY [J].
HOY, JL ;
MACAULEY, BJ ;
FINCHER, GB .
JOURNAL OF THE INSTITUTE OF BREWING, 1981, 87 (02) :77-80