Staphylococcus aureus Small Colony Variants (SCVs): News From a Chronic Prosthetic Joint Infection

被引：73

作者：

Loss, Guilherme ^{[1
]}

Simoes, Patricia Martins ^{[2
,3
]}

Valour, Florent ^{[3
,4
]}

Cortes, Marina Farrel ^{[5
]}

Gonzaga, Luiz ^{[1
]}

Bergot, Marine ^{[3
]}

Trouillet-Assant, Sophie ^{[6
]}

Josse, Jerome ^{[3
]}

Diot, Alan ^{[3
]}

Ricci, Emiliano ^{[3
]}

Vasconcelos, Ana Tereza ^{[1
]}

Laurent, Frederic ^{[2
,3
]}

机构：

[1] Lab Nacl Comp Cient, Rio De Janeiro, Brazil

[2] Northern Hosp Grp, IAI Dept Clin Microbiol, Hosp Civils Lyon, Natl Reference Ctr Staphylococci, Lyon, France

[3] CIRI, Lyon, France

[4] Northern Hosp Grp, Infect Dis Dept, Hosp Civils Lyon, Lyon, France

[5] Univ Fed Rio de Janeiro, Inst Microbiol Prof Paulo de Goes, Rio De Janeiro, Brazil

[6] Ctr Hosp Lyon Sud, Joint Res Unit HCL BioMerieux, Hosp Civils Lyon, Pierre Benite, France

来源：

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY | 2019年 / 9卷

关键词：

Staphylococcus aureus; small colony variant; prosthetic joint infection; chronic infection; rifampicin resistance; DIFFERENTIAL EXPRESSION ANALYSIS; ANTIMICROBIAL ACTIVITY; RIFAMPIN RESISTANCE; STRINGENT RESPONSE; OSTEOMYELITIS; PERSISTENT; VIRULENCE; BACTERIA; MUTATION; IRON;

D O I：

10.3389/fcimb.2019.00363

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Small colony variants (SCV) of Staphylococcus aureus have been reported as implicated in chronic infections. Here, we investigated the genomic and transcriptomic changes involved in the evolution from a wild-type to a SCV from in a patient with prosthetic joint infection relapse. The SCV presented a stable phenotype with no classical auxotrophy and the emergence of rifampicin resistance. Whole Genome Sequencing (WGS) analysis showed only the loss of a 42.5 kb phage and 3 deletions, among which one targeting the rpoB gene, known to be the target of rifampicin and to be associated to SCV formation in the context of a constitutively active stringent response. Transcriptomic analysis highlighted a specific signature in the SCV strain including a complex, multi-level strategy of survival and adaptation to chronicity within the host including a protection from the inflammatory response, an evasion of the immune response, a constitutively activated stringent response and a scavenging of iron sources.

引用

页数：12

共 72 条

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