Recent Advances in Droplet-based Microfluidic Technologies for Biochemistry and Molecular Biology

被引：70

作者：

Barea, Joel Sanchez ^{[1
]}

Lee, Juhwa ^{[1
]}

Kang, Dong-Ku ^{[2
]}

机构：

[1] Incheon Natl Univ, Dept Chem, Incheon 22012, South Korea

[2] Incheon Natl Univ, Res Inst Basic Sci, Dept Chem, Incheon 22012, South Korea

来源：

MICROMACHINES | 2019年 / 10卷 / 06期

关键词：

droplet-based microfluidic; biochemistry; molecular biology; digital PCR; biochip; biosensor; digital quantification; microfluidic; single cell analysis; MEDIATED ISOTHERMAL AMPLIFICATION; POLYMERASE-CHAIN-REACTION; SINGLE-CELL ANALYSIS; HIGH-THROUGHPUT; ABSOLUTE QUANTIFICATION; DIGITAL PCR; QUANTITATIVE DETECTION; CHIP; PLATFORM; CANCER;

D O I：

10.3390/mi10060412

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Recently, droplet-based microfluidic systems have been widely used in various biochemical and molecular biological assays. Since this platform technique allows manipulation of large amounts of data and also provides absolute accuracy in comparison to conventional bioanalytical approaches, over the last decade a range of basic biochemical and molecular biological operations have been transferred to drop-based microfluidic formats. In this review, we introduce recent advances and examples of droplet-based microfluidic techniques that have been applied in biochemistry and molecular biology research including genomics, proteomics and cellomics. Their advantages and weaknesses in various applications are also comprehensively discussed here. The purpose of this review is to provide a new point of view and current status in droplet-based microfluidics to biochemists and molecular biologists. We hope that this review will accelerate communications between researchers who are working in droplet-based microfluidics, biochemistry and molecular biology.

引用

页数：25

共 154 条

[81] Picoliter Well Array Chip-Based Digital Recombinase Polymerase Amplification for Absolute Quantification of Nucleic Acids [J].

Li, Zhao ;

Liu, Yong ;

Wei, Qingquan ;

Liu, Yuanjie ;

Liu, Wenwen ;

Zhang, Xuelian ;

Yu, Yude .

PLOS ONE, 2016, 11 (04)

[82] 3D-Printed High-Density Droplet Array Chip for Miniaturized Protein Crystallization Screening under Vapor Diffusion Mode [J].

Liang, Yi-Ran ;

Zhu, Li-Na ;

Gao, Jie ;

Zhao, Hong-Xia ;

Zhu, Ying ;

Ye, Sheng ;

Fang, Qun .

ACS APPLIED MATERIALS & INTERFACES, 2017, 9 (13) :11837-11845

[83] PCR-Activated Cell Sorting for Cultivation-Free Enrichment and Sequencing of Rare Microbes [J].

Lim, Shaun W. ;

Tran, Tuan M. ;

Abate, Adam R. .

PLOS ONE, 2015, 10 (01)

[84] Clinical relevance of circulating MACC1 and S100A4 transcripts for ovarian cancer [J].

Link, Theresa ;

Kuhlmann, Jan Dominik ;

Kobelt, Dennis ;

Herrmann, Pia ;

Vassileva, Yana D. ;

Kramer, Michael ;

Frank, Kerstin ;

Goeckenjan, Maren ;

Wimberger, Pauline ;

Stein, Ulrike .

MOLECULAR ONCOLOGY, 2019, 13 (05) :1268-1279

[85] Single-Exosome-Counting Immunoassays for Cancer Diagnostics [J].

Liu, Chunchen ;

Xu, Xiaonan ;

Li, Bo ;

Situ, Bo ;

Pan, Weilun ;

Hu, Yu ;

An, Taixue ;

Yao, Shuhuai ;

Zheng, Lei .

NANO LETTERS, 2018, 18 (07) :4226-4232

[86] Thermoplastic microfluidic devices and their applications in protein and DNA analysis [J].

Liu, Ke ;

Fan, Z. Hugh .

ANALYST, 2011, 136 (07) :1288-1297

[87] Integrated microfluidic systems for high-performance genetic analysis [J].

Liu, Peng ;

Mathies, Richard A. .

TRENDS IN BIOTECHNOLOGY, 2009, 27 (10) :572-581

[88] Quantitative Analysis of HER2 Amplification by Droplet Digital PCR in the Follow-Up of Gastric Cancer Patients Being Treated with Trastuzumab after Surgery [J].

Liu, Yanzhuo ;

Yang, Maozhu ;

Jiang, Tao ;

Lan, Chunbin ;

Yuan, Hao ;

Li, Guiquan ;

Jia, Guiqing ;

Wang, Kang ;

Zhao, Gaoping .

GASTROENTEROLOGY RESEARCH AND PRACTICE, 2019, 2019

[89] Detecting and Tracking Nosocomial Methicillin-Resistant Staphylococcus aureus Using a Microfluidic SERS Biosensor [J].

Lu, Xiaonan ;

Samuelson, Derrick R. ;

Xu, Yuhao ;

Zhang, Hongwei ;

Wang, Shuo ;

Rasco, Barbara A. ;

Xu, Jie ;

Konkel, Michael E. .

ANALYTICAL CHEMISTRY, 2013, 85 (04) :2320-2327

[90] Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform [J].

Ma, Fuqiang ;

Chung, Meng Ting ;

Yao, Yuan ;

Nidetz, Robert ;

Lee, Lap Man ;

Liu, Allen P. ;

Feng, Yan ;

Kurabayashi, Katsuo ;

Yang, Guang-Yu .

NATURE COMMUNICATIONS, 2018, 9

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