Multifocus microscopy with precise color multi-phase diffractive optics applied in functional neuronal imaging

被引：43

作者：

Abrahamsson, Sara ^{[1
,2
,3
]}

Ilic, Rob ^{[3
]}

Wisniewski, Jan ^{[4
]}

Mehl, Brian ^{[4
]}

Yu, Liya ^{[3
]}

Chen, Lei ^{[3
]}

Davanco, Marcelo ^{[3
]}

Oudjedi, Laura ^{[5
]}

Fiche, Jean-Bernard ^{[5
]}

Hajj, Bassam ^{[4
]}

Jin, Xin ^{[1
,2
]}

Pulupa, Joan ^{[6
]}

Cho, Christine ^{[1
,2
]}

Mir, Mustafa ^{[4
,7
]}

El Beheiry, Mohamed ^{[4
,8
]}

Darzacq, Xavier ^{[4
,7
]}

Nollmann, Marcelo ^{[5
]}

Dahan, Maxime ^{[4
,8
]}

Wu, Carl ^{[4
]}

Lionnet, Timothee ^{[4
]}

Liddle, J. Alexander ^{[3
]}

Bargmann, Cornelia I. ^{[1
,2
]}

机构：

[1] Rockefeller Univ, HHMI, New York, NY 10065 USA

[2] Rockefeller Univ, Lulu & Anthony Wang Lab Neural Circuits & Behav, New York, NY 10065 USA

[3] NIST, Ctr Nanoscale Sci & Technol, Gaithersburg, MD 20899 USA

[4] Transcript Imaging Consortium, HHMI Janelia Res Campus, Ashburn, VA 20147 USA

[5] Univ Montpellier, INSERM U1054, CNRS UMR5048, Ctr Biochim Struct, 29 Rue Navacelles, F-34090 Montpellier, France

[6] Rockefeller Univ, Lab Cellular Biophys, New York, NY 10065 USA

[7] Univ Calif Berkeley, Berkeley, CA 94720 USA

[8] Univ Paris 06, Inst Curie, CNRS UMR 168, Lab Physico Chim, 11 Rue Pierre & Marie Curie, F-75005 Paris, France

来源：

BIOMEDICAL OPTICS EXPRESS | 2016年 / 7卷 / 03期

基金：

欧洲研究理事会;

关键词：

CELL;

D O I：

10.1364/BOE.7.000855

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Multifocus microscopy (MFM) allows high-resolution instantaneous three-dimensional (3D) imaging and has been applied to study biological specimens ranging from single molecules inside cells nuclei to entire embryos. We here describe pattern designs and nanofabrication methods for diffractive optics that optimize the light-efficiency of the central optical component of MFM: the diffractive multifocus grating (MFG). We also implement a "precise color" MFM layout with MFGs tailored to individual fluorophores in separate optical arms. The reported advancements enable faster and brighter volumetric time-lapse imaging of biological samples. In live microscopy applications, photon budget is a critical parameter and light-efficiency must be optimized to obtain the fastest possible frame rate while minimizing photodamage. We provide comprehensive descriptions and code for designing diffractive optical devices, and a detailed methods description for nanofabrication of devices. Theoretical efficiencies of reported designs is approximate to 90% and we have obtained efficiencies of > 80% in MFGs of our own manufacture. We demonstrate the performance of a multi-phase MFG in 3D functional neuronal imaging in living C. elegans. (C) 2016 Optical Society of America

引用

页码：855 / 869

页数：15

共 23 条

[1]

Abrahamsson S., 2016, MFM FUNCTIONAL NEURO

[2]

Abrahamsson S., 2015, DESIGN YOUR OWN DIFF

[3] MultiFocus Polarization Microscope (MF-PolScope) for 3D polarization imaging of up to 25 focal planes simultaneously [J].

Abrahamsson, Sara ;

McQuilken, Molly ;

Mehta, Shalin B. ;

Verma, Amitabh ;

Larsch, Johannes ;

Ilic, Rob ;

Heintzmann, Rainer ;

Bargmann, Cornelia I. ;

Gladfelter, Amy S. ;

Oldenbourg, Rudolf .

OPTICS EXPRESS, 2015, 23 (06) :7734-7754

[4]

Abrahamsson S, 2013, NAT METHODS, V10, P60, DOI [10.1038/nmeth.2277, 10.1038/NMETH.2277]

[5] Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging [J].

Akerboom, Jasper ;

Chen, Tsai-Wen ;

Wardill, Trevor J. ;

Tian, Lin ;

Marvin, Jonathan S. ;

Mutlu, Sevinc ;

Calderon, Nicole Carreras ;

Esposti, Federico ;

Borghuis, Bart G. ;

Sun, Xiaonan Richard ;

Gordus, Andrew ;

Orger, Michael B. ;

Portugues, Ruben ;

Engert, Florian ;

Macklin, John J. ;

Filosa, Alessandro ;

Aggarwal, Aman ;

Kerr, Rex A. ;

Takagi, Ryousuke ;

Kracun, Sebastian ;

Shigetomi, Eiji ;

Khakh, Baljit S. ;

Baier, Herwig ;

Lagnado, Leon ;

Wang, Samuel S. -H. ;

Bargmann, Cornelia I. ;

Kimmel, Bruce E. ;

Jayaraman, Vivek ;

Svoboda, Karel ;

Kim, Douglas S. ;

Schreiter, Eric R. ;

Looger, Loren L. .

JOURNAL OF NEUROSCIENCE, 2012, 32 (40) :13819-13840

[6]

[Anonymous], 2013, Patent Application, Patent No. 20130176622

[7] Simultaneous multiplane imaging with a distorted diffraction grating [J].

Blanchard, PM ;

Greenaway, AH .

APPLIED OPTICS, 1999, 38 (32) :6692-6699

[8]

Born M., 2002, PRINCIPLES OPTICS, P446

[9] Wave optics theory and 3-D deconvolution for the light field microscope [J].

Broxton, Michael ;

Grosenick, Logan ;

Yang, Samuel ;

Cohen, Noy ;

Andalman, Aaron ;

Deisseroth, Karl ;

Levoy, Marc .

OPTICS EXPRESS, 2013, 21 (21) :25418-25439

[10] Microfluidics for in vivo imaging of neuronal and behavioral activity in Caenorhabditis elegans [J].

Chronis, Nikos ;

Zimmer, Manuel ;

Bargmann, Cornelia I. .

NATURE METHODS, 2007, 4 (09) :727-731

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