Effect of traditional Chinese medicine formula Sinisan on chronic restraint stressinduced nonalcoholic fatty liver disease: a rat study

被引:36
作者
Cheng, Fafeng [1 ]
Ma, Chongyang [1 ]
Wang, Xueqian [1 ]
Zhai, Changming [1 ]
Wang, Guoli [1 ]
Xu, Xiaolin [1 ]
Mu, Jie [1 ]
Li, Changxiang [1 ]
Wang, Zisong [1 ]
Zhang, Xiaoyu [1 ]
Yue, Wenchao [1 ]
Du, Xin [1 ]
Lian, Yajun [1 ]
Zhu, Wenxiang [1 ]
Yin, Xiangjun [1 ]
Wei, Zhen [1 ]
Song, Wenjie [1 ]
Wang, Qingguo [1 ]
机构
[1] Beijing Univ Chinese Med, Sch Basic Med Sci, 11 Beisanhuandong Rd, Beijing 100029, Peoples R China
来源
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2017年 / 17卷
关键词
Psychological stress; Chronic stress; NAFLD; TCM; Sinisan; Oxidative stress; SI-NI-SAN; OXIDATIVE STRESS; 4; PRESCRIPTIONS; STEATOHEPATITIS; MODEL; DIET; INJURY; NAFLD; IDENTIFICATION; ACIDS;
D O I
10.1186/s12906-017-1707-2
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
Background: Nonalcoholic fatty liver disease (NAFLD) represents one of the most common forms of liver disease worldwide, and it is always regarded as a consequence of a sedentary, food-abundant lifestyle, sitting for an extended time, and a low physical activity level, which often coincide with chronic and long-lasting psychological stress. A Chinese medicine Sinisan (SNS) may be a potential formula for treating this kind of disease. Methods: In this study, a long-term chronic restraint stress protocol was used to investigate the mechanism underlying stress-induced NALFD. To investigate the effect of SNS treatment on stress-induced NAFLD, we measured the liver and serum values of total cholesterol (TC), triglyceride (TG), liver free fatty acids (FFA), low-density lipoprotein, superoxide dismutase, tumor necrosis factor-alpha, malondialdehyde, interleukin (IL)-6, and serum values of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase. Results are shown as a mean +/- standard deviation. Significant differences between the groups were evaluated using the Student t-test. For multiple comparisons, one-way analysis of variance (ANOVA) was used. If the results of ANOVA indicated significant differences, post hoc analysis was performed with the Tukey test or Dunnett test, and p < 0.05 was considered statistically significant. Results: Long-term chronic stress led to steatosis and non-alcoholic steatohepatitis. Additionally, SNS treatment significantly increased body weight gain (p < 0.01) and sucrose preference (p < 0.001), and it reduced the liver values of TC, TG, and FFA (p < 0.05). SNS also reduced the serum values of AST and ALT (p < 0.001), and the liver value of IL-6 (p < 0.01). Conclusions: This study's results demonstrate that psychological stress may be a significant risk factor of NAFLD. Furthermore, the traditional Chinese medicine formula SNS may have some beneficial effect in antagonizing psychological stress and stress-related NAFLD.
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页数:10
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